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SECTION 5 

– CONCENTRATION MEASUREMENTS 

The  concentration  measurement  mode  enables  simple  measurements  of  concentration  to  be 

performed.  In  this  measurement  mode  it  is  possible  to  calibrate  against  a  standard  of  a  known 

concentration or use a known factor. The sample is measured at one wavelength at one point in time.  

To select concentration use the LEFT or RIGHT arrow keys to move the cursor to the CONC indicator 

on  the  menu  bar  (refer  to  figure  2.3.1).  The  primary  display  will  show  the  concentration  with  the 

selected units. To adjust the wavelength use the UP and DOWN arrow keys. 

To select the units use the LEFT or RIGHT arrow keys to move the cursor to the  UNITS indicator on 

the menu bar (refer to figure 2.3.1). Use the UP and DOWN arrow keys to select ppm, mg/l, g/l, M, % 

or blank units. Once the required units have been selected  press the RETURN  or CAL key to store 

the units and return to the concentration measurement mode.  

5.1 

USING A KNOWN STANDARD 

The  calibration  must  be  performed  at  the  same  wavelength  at  which  the  sample  will  be  measured. 

Insert a cuvette containing the known concentration standard into the sample chamber and close the 

instrument lid. Press the CAL key; the CAL annunciator will flash continuously. Use the arrow keys to 

change  the  concentration  to  the  required  value  for  the  standard.  Press  the  CAL  or  RETURN  key  to 

calibrate  the  instrument  to  the  standard  concentration.  The  instrument  will  calculate  the  factor  for 

subsequent measurements. If the factor calculated is out of range, then the instrument will display Err 

3 for 2 seconds after the calibration.  To view the factor use the LEFT or RIGHT arrow keys to move 

the cursor to the xF indicator on the menu bar (refer to figure 2.3.1). 

Once  the  calibration  has  been  performed  a  sample  of  unknown  concentration  can  be  measured. 

Remove the cuvette containing the standard solution and place a cuvette containing the sample to be 

measured in the sample holder. Close the instrument lid and the concentration result will be shown on 

the screen.  

Subsequent  samples  can  be  measured  in  the  same  way.  If  the  wavelength  is  adjusted  between 

sample  measurements  then  the  instrument  must  be  calibrated  again  before  more  samples  can  be 

measured. 

5.2 

USING A KNOWN FACTOR   

If the factor is known there is no need to calibrate against a known standard. To enter the factor use 

the LEFT or RIGHT arrow keys to move the cursor to the xF indicator on the menu bar (refer to figure 

2.3.1). Use the UP and DOWN arrow keys to enter the required factor. Once the required factor has 

been  entered  press  the  RETURN  or  CAL  key  to  store  the  factor  and  return  to  the  concentration 

measurement mode.  

Содержание 6305

Страница 1: ...Operating Manual 635 015 REV B 06 14 Model 6305 Spectrophotometer...

Страница 2: ...2...

Страница 3: ...val of covers and attempted adjustment or service by unqualified personnel will invalidate any warranty and incur additional charges for repair 7 Reference should always be made to the Health and Safe...

Страница 4: ...n de ces produits chimiques doivent tre respect s soigneusement Lorsqu elles ne sont pas disponibles ces donn es doivent tre demand es dans tous les cas au fournisseur avant de poursuivre 8 Il est imp...

Страница 5: ...eine geeignete Sicherheitsausr stung und pers nliche Schutzausr stung zu verwenden 9 Falls vermutet wird dass der Sicherheitsschutz auf irgendeine Weise beeintr chtigt wurde ist das Spektrophotometer...

Страница 6: ...are o utilizzare lo strumento 1 Gli spettrofotometri della serie 63 sono progettati per essere utilizzati da personale qualificato che conosce i principi e le applicazioni ad essi collegati Per ulteri...

Страница 7: ...tiche di laboratorio quando si manipolano campioni sostanze chimiche reagenti e apparecchiature accessorie per eseguire misurazioni e analisi con questo strumento Attrezzature idonee per la protezione...

Страница 8: ...RICS MEASUREMENTS 18 4 1 TRANSMISSION MEASUREMENT MODE 18 4 2 ABSORBANCE MEASUREMENT MODE 18 SECTION 5 CONCENTRATION MEASUREMENTS 20 5 1 USING A KNOWN STANDARD 20 5 2 USING A KNOWN FACTOR 20 SECTION 6...

Страница 9: ...ontamination Combined with a mechanically rigid structure this model provides a system with fast warm up low drift and high reliability 1 2 INSTRUMENT SPECIFICATION 6305 Wavelength Range 198 to 1000nm...

Страница 10: ...he mains fuse is housed within the POWER IN socket When replacing the fuse the unit must be disconnected from the mains supply The fuse rating is 2A F fast blow type In the event of the fuse failing a...

Страница 11: ...g 2 3 1 Display 1 Primary display area Transmission Absorbance Concentration 2 Primary display adjust annunciator 3 Secondary display area Wavelength Factor 4 Primary display units 5 Secondary display...

Страница 12: ...u option 4 CAL Key Initiates a calibration routine 5 Print Key Provides a printout of the current reading with an incremental sample number When pressed for the first time after a calibration the prin...

Страница 13: ...ess panel and all ventilation slots must NOT be covered or obstructed at any time 2 Power switch On off switch for the unit 3 Power in socket IEC type connection socket for mains cable 4 Output socket...

Страница 14: ...is travelling through The Beer Lambert law states that A is the absorbance is the molar absorption coefficient l mol 1 cm 1 c is the concentration mol l 1 l is the path length cm This law shows that...

Страница 15: ...enon lamp is focused onto the grating with 1200 lines per millimeter which separates the light into discreet wavelengths The diffracted spectrum of light then passes through a further slit and lens ar...

Страница 16: ...to 260nm Below this level quartz cuvettes must be used NOTE the plastic cuvettes supplied with the unit are not suitable for use below a wavelength of 320nm b Plastic disposable cuvettes should only b...

Страница 17: ...In the preparation of samples and standards high grade borosilicate glass and AR grade chemicals and reagents must be used Good quality deionised water or other suitable solvents must be used for diss...

Страница 18: ...screen Subsequent samples can be measured in the same way If the wavelength is adjusted between sample measurements then the instrument must be calibrated again before more samples can be measured 4 2...

Страница 19: ...19 measured...

Страница 20: ...ously Use the arrow keys to change the concentration to the required value for the standard Press the CAL or RETURN key to calibrate the instrument to the standard concentration The instrument will ca...

Страница 21: ...also be sent to the 6305 via the serial interface using Windows HyperTerminal for example ASCII D or d Same as pressing the PRINT key ASCII T CR Outputs transmission and wavelength separated by an AS...

Страница 22: ...readily incorporated into most spreadsheet software packages 6 1 RS232 Output The bi directional RS232 interface is available on the rear panel 25 way D type connector The connections are as follows T...

Страница 23: ...PC GND 7 5 GND Note the interface cable kit 542 009 can be used to implement the above interconnections 6 2 Recorder Output This is available via the 4mm rear panel sockets The level is proportional...

Страница 24: ...or use with computer without a serial port 543 001 40 Column printer supplied with interface cable paper roll and ribbon and universal power supply 060 287 Paper roll for printer quantity 1 060 288 Pr...

Страница 25: ...or commercial use and will give rise to errors if used 8 2 LAMP REPLACEMENT 8 2 1 Xenon Flash Lamp Module Replacement Before replacing the lamp disconnect the unit from the mains supply Access to the...

Страница 26: ...26...

Страница 27: ...e Department for a reference number which you should include with your faulty equipment Please also ensure you include a clear description of the fault and a completed copy of our Decontamination Cert...

Страница 28: ...is outside the instrument range Recalibrate the instrument using a standard of lower concentration Err 5 Lamp Failure The instrument is unable to detect a peak signal for wavelength calibration Possi...

Страница 29: ...29 SECTION 10 Declaration of Conformity...

Страница 30: ...748 Fax 33 1 64 45 13 00 Fax 44 0 1785 810405 e mail info bibby scientific com e mail bsf bibby scientific fr e mail bibby bibby scientificasia com www bibby scientific com www bibby scientific com ww...

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