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16 

3.3         GOOD PRACTICE GUIDELINES 

 

 

1.  For optimum performance all spectrophotometers should be sited in a clean, dry, dust 

free atmosphere. When in use ambient temperature and light levels should remain as 

constant as possible. 

2.  If  required  adherence  to  Standard  Operating  Procedures  (SOP)  and  Good  Laboratory 

Practice (GLP) should be monitored with regular calibration checks and a suitable Quality 

Control (QC) programme. 

3.  The  sample  chamber  lid  must  be  fully  closed  during  measurement  and  before  any 

readings are recorded or printed. 

4.  The  correct  selection  of  sample  containers  is  imperative  for  accurate  and  reproducible 

results: 

a)  Check that the material of the sample container is compatible with the wavelengths to 

be  used  for  measurement.  In  general  glass  can  only  be  used  down  to  360nm  or 

320nm depending on quality. Standard plastic cuvettes can be used down to 320nm. 

Special  UV  versions  can  be  used  down  to  260nm.  Below  this  level  quartz  cuvettes 

must be used. 

NOTE:

 

the plastic cuvettes supplied with the unit are not suitable 

for use below a wavelength of 320nm.

  

b)  Plastic disposable cuvettes should only be used ONCE. 

c)  Glass  cuvettes  should  be  thoroughly  cleaned  after  use.  Discard  when  scratches 

become evident on optical surfaces. 

d)  Care  should  be  taken  when  selecting  semi-micro  or  micro  cuvettes.  The  cuvette 

window  on  the  inner  chamber  (the  area  filled  with  sample)  must  be  wider  than  the 

aperture in the sample holder or light will reach the detector without passing through 

the  sample.  In  this  case,  semi-micro  or  micro  cuvettes  with  self-screening  black 

surrounds must be used or, alternative holders for these cuvettes should be used. 

e)  Glass test tubes and other sample tubes should be used with care. Where possible, 

matched tubes should be used and any index mark set to the correct position before 

measurements are made. 

f)  Ensure any sample containers used are compatible with the constituents of both the 

samples  and  standards  they  are  to  hold.  Plastic  cuvettes  are  not  compatible  with 

organic solvents. 

Содержание 6305

Страница 1: ...Operating Manual 635 015 REV B 06 14 Model 6305 Spectrophotometer...

Страница 2: ...2...

Страница 3: ...val of covers and attempted adjustment or service by unqualified personnel will invalidate any warranty and incur additional charges for repair 7 Reference should always be made to the Health and Safe...

Страница 4: ...n de ces produits chimiques doivent tre respect s soigneusement Lorsqu elles ne sont pas disponibles ces donn es doivent tre demand es dans tous les cas au fournisseur avant de poursuivre 8 Il est imp...

Страница 5: ...eine geeignete Sicherheitsausr stung und pers nliche Schutzausr stung zu verwenden 9 Falls vermutet wird dass der Sicherheitsschutz auf irgendeine Weise beeintr chtigt wurde ist das Spektrophotometer...

Страница 6: ...are o utilizzare lo strumento 1 Gli spettrofotometri della serie 63 sono progettati per essere utilizzati da personale qualificato che conosce i principi e le applicazioni ad essi collegati Per ulteri...

Страница 7: ...tiche di laboratorio quando si manipolano campioni sostanze chimiche reagenti e apparecchiature accessorie per eseguire misurazioni e analisi con questo strumento Attrezzature idonee per la protezione...

Страница 8: ...RICS MEASUREMENTS 18 4 1 TRANSMISSION MEASUREMENT MODE 18 4 2 ABSORBANCE MEASUREMENT MODE 18 SECTION 5 CONCENTRATION MEASUREMENTS 20 5 1 USING A KNOWN STANDARD 20 5 2 USING A KNOWN FACTOR 20 SECTION 6...

Страница 9: ...ontamination Combined with a mechanically rigid structure this model provides a system with fast warm up low drift and high reliability 1 2 INSTRUMENT SPECIFICATION 6305 Wavelength Range 198 to 1000nm...

Страница 10: ...he mains fuse is housed within the POWER IN socket When replacing the fuse the unit must be disconnected from the mains supply The fuse rating is 2A F fast blow type In the event of the fuse failing a...

Страница 11: ...g 2 3 1 Display 1 Primary display area Transmission Absorbance Concentration 2 Primary display adjust annunciator 3 Secondary display area Wavelength Factor 4 Primary display units 5 Secondary display...

Страница 12: ...u option 4 CAL Key Initiates a calibration routine 5 Print Key Provides a printout of the current reading with an incremental sample number When pressed for the first time after a calibration the prin...

Страница 13: ...ess panel and all ventilation slots must NOT be covered or obstructed at any time 2 Power switch On off switch for the unit 3 Power in socket IEC type connection socket for mains cable 4 Output socket...

Страница 14: ...is travelling through The Beer Lambert law states that A is the absorbance is the molar absorption coefficient l mol 1 cm 1 c is the concentration mol l 1 l is the path length cm This law shows that...

Страница 15: ...enon lamp is focused onto the grating with 1200 lines per millimeter which separates the light into discreet wavelengths The diffracted spectrum of light then passes through a further slit and lens ar...

Страница 16: ...to 260nm Below this level quartz cuvettes must be used NOTE the plastic cuvettes supplied with the unit are not suitable for use below a wavelength of 320nm b Plastic disposable cuvettes should only b...

Страница 17: ...In the preparation of samples and standards high grade borosilicate glass and AR grade chemicals and reagents must be used Good quality deionised water or other suitable solvents must be used for diss...

Страница 18: ...screen Subsequent samples can be measured in the same way If the wavelength is adjusted between sample measurements then the instrument must be calibrated again before more samples can be measured 4 2...

Страница 19: ...19 measured...

Страница 20: ...ously Use the arrow keys to change the concentration to the required value for the standard Press the CAL or RETURN key to calibrate the instrument to the standard concentration The instrument will ca...

Страница 21: ...also be sent to the 6305 via the serial interface using Windows HyperTerminal for example ASCII D or d Same as pressing the PRINT key ASCII T CR Outputs transmission and wavelength separated by an AS...

Страница 22: ...readily incorporated into most spreadsheet software packages 6 1 RS232 Output The bi directional RS232 interface is available on the rear panel 25 way D type connector The connections are as follows T...

Страница 23: ...PC GND 7 5 GND Note the interface cable kit 542 009 can be used to implement the above interconnections 6 2 Recorder Output This is available via the 4mm rear panel sockets The level is proportional...

Страница 24: ...or use with computer without a serial port 543 001 40 Column printer supplied with interface cable paper roll and ribbon and universal power supply 060 287 Paper roll for printer quantity 1 060 288 Pr...

Страница 25: ...or commercial use and will give rise to errors if used 8 2 LAMP REPLACEMENT 8 2 1 Xenon Flash Lamp Module Replacement Before replacing the lamp disconnect the unit from the mains supply Access to the...

Страница 26: ...26...

Страница 27: ...e Department for a reference number which you should include with your faulty equipment Please also ensure you include a clear description of the fault and a completed copy of our Decontamination Cert...

Страница 28: ...is outside the instrument range Recalibrate the instrument using a standard of lower concentration Err 5 Lamp Failure The instrument is unable to detect a peak signal for wavelength calibration Possi...

Страница 29: ...29 SECTION 10 Declaration of Conformity...

Страница 30: ...748 Fax 33 1 64 45 13 00 Fax 44 0 1785 810405 e mail info bibby scientific com e mail bsf bibby scientific fr e mail bibby bibby scientificasia com www bibby scientific com www bibby scientific com ww...

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