Table 5 NGS library size selection reference
(continued)
Library Size
Target library
peak
Run time to
reference line
Input sample
amount
Stop the run
and collect your
sample when…
Schematic view
100-base-read
200 bp
11–12.5 min
50–100 ng
200 bp band is
in the
middle
of
the
collection
well
Ion Proton
™
System
200-base-read
270 bp
12–14 min
500 ng
300 bp band is
at the
top
of the
exposed
agarose area
50–100 ng
300 bp band is
at the
middle
of
the
exposed
agarose area
150-base-read
220 bp
11–14.5 min
500 ng
250 bp band is
at the
middle
of
the
exposed
agarose area
50–100 ng
250 bp band is
at the
middle
of
the
exposed
agarose area
Quantitation of isolated DNA
• Recovered DNA can be assessed using the Qubit
™
fluorometer (Cat. no. Q32868), or by gel
electrophoresis.
• qPCR is recommended for accurate quantitation of next generation sequencing libraries recovered
from E
‑
Gel
™
SizeSelect
™
II gels.
• Recovered samples are not compatible with 280 nm measurements without first performing buffer
exchange.
Appendix E
E
‑
Gel
™
SizeSelect
™
II Agarose Gels
Quantitation of isolated DNA
E
E
‑
Gel
™
Power Snap Plus Electrophoresis System User Guide
67
