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Invitrogen E-Gel Power Snap Plus, Руководство пользователя

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Invitrogen E-Gel Power Snap Plus Скачать руководство пользователя страница 27

Prepare the gel

1.

Remove the gel from the package.

2.

Gently remove the combs. Do not allow the combs to bend or create suction in the wells during
removal.

3.

Place the cassette into a E

‑

Gel

™

 Adapter.

4.

Insert the gel cassette and adapter into the E

‑

Gel

™

 Power Snap Plus Electrophoresis Device,

starting from the right edge.

5.

Press down on the left side of the cassette to secure it into the device.

6.

Load gels within 15 minutes after opening the package.

Sample loading guidelines

•

Use the recommended total loading volume for each gel type.

 Do not load more than

recommended amount of DNA sample or ladder per well.

•

Load deionized water into all empty wells.

•

Keep all sample volumes uniform.

 If you do not have enough samples to load all the wells of

the gel, load an identical volume of deionized water into any empty wells. Prepare your samples
by adding E

‑

Gel

™

 1X Sample Loading Buffer or deionized water to the required amount of DNA to

bring the total required sample volume.

• Avoid introducing bubbles while loading. Bubbles can cause band distortion.

Gel type

Total loading volume

E

‑

Gel

™

 EX

20 µL

E

‑

Gel

™

 EX Double Comb

E

‑

Gel

™

 with SYBR

™

 Safe

E

‑

Gel

™

 Double Comb with SYBR

™

 Safe

E

‑

Gel

™

 48 Agarose Gel with SYBR

™

 Safe

15 µL

E

‑

Gel

™

 96 Agarose Gel with SYBR

™

 Safe

20 µL

E

‑

Gel

™

 CloneWell

™

 II

25 µL

E

‑

Gel

™

 SizeSelect

™

 II

25 µL

E

‑

Gel

™

 NGS

20 µL

E-PAGE

™

 48

15 µL

E-PAGE

™

 96

20 µL

Chapter 2 

Methods

Using the E

‑

Gel

™

 Power Snap Plus Electrophoresis Device

2

E

‑

Gel

™

 Power Snap Plus Electrophoresis System User Guide

27

Содержание E-Gel Power Snap Plus

Страница 1: ...dures E Gel Power Snap Plus Electrophoresis System USER GUIDE For use with E Gel E Gel EX E Gel 48 and E Gel 96 Agarose Gels and E PAGE Gels Catalog Numbers G9100 G9200 G9101 G9201 G9301 G9341 G9342 G...

Страница 2: ...IPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING YOUR USE OF IT Revision history Pub No MAN0002591 Revision Date Description A 0 5 April 2022 New user guide for...

Страница 3: ...rst time instrument setup 12 Set up the base module 12 Set up the camera module 12 CHAPTER 2 Methods 24 Using the E Gel Power Snap Plus Electrophoresis Device 24 User interface overview 24 Required ma...

Страница 4: ...strument settings 46 Maintenance services 50 APPENDIX C E Gel agarose gels 51 Nucleic acid stains used in E Gel agarose gels 51 SYBR Safe DNA gel stain 51 SYBR gold II gel stain 52 Opening E Gel casse...

Страница 5: ...products 70 Choosing the right gel 70 E Gel Agarose Gels 72 Choosing the right DNA ladder 74 Ladders and buffers 75 Accessory items 75 APPENDIX H Safety 76 Before starting 76 Installing the instrument...

Страница 6: ...APPENDIX I Documentation and support 85 Customer and technical support 85 Limited product warranty 85 Contents 6 E Gel Power Snap Plus Electrophoresis System User Guide...

Страница 7: ...Gel EX E Gel 48 and E Gel 96 agarose gels as well as E PAGE gels Product contents Base module kit contents The contents of the E Gel Power Snap Plus Electrophoresis Device kit are listed in the follo...

Страница 8: ...Gel Power Snap Plus Camera 1 System components Parts of the E Gel Power Snap Plus Base Module and AC DC power adapter 1 2 4 3 5 6 7 7 8 9 10 11 1 E Gel cassette compartment 2 Blue light transilluminat...

Страница 9: ...4 Battery compartment Rear view of the E Gel Power Snap Plus camera module and base module 6 3 4 2 6 5 1 1 USB port for wireless module 2 Ethernet port 3 DC input port 4 Power switch 5 USB port for im...

Страница 10: ...of the camera module Table 2 Status indicators Button Function 1 4 View instrument status 1 Time remaining 2 Protocol status step paused etc Indicates whether a USB device is inserted into the instru...

Страница 11: ...on or other symbols 4 Delete 5 Close and save 6 Close without saving Enter numbers When you press a field that requires a numerical input the numeric editor as seen in the following figure opens 2 3 4...

Страница 12: ...user to set instrument parameters before use and to access cloud based resources Set up the base module 1 Remove the protective film covering the amber filter 2 Connect the power cable to the power ad...

Страница 13: ...Network configuration screen 2 In the Network configuration screen select a field in the Wireless panel 1 2 1 Wireless panel 2 Wired panel Note During initial setup if you selected the Wired option i...

Страница 14: ...ou have selected enter the appropriate passwords and select Join 8 If all the entered information is correct the Network Connection Complete screen will appear Select OK to continue Note If incorrect...

Страница 15: ...t Settings screen select Network configuration 4 In the Network Connection screen select a field in the Wired panel 1 2 1 Wireless panel 2 Wired panel Chapter 1 Product information First time instrume...

Страница 16: ...f X X X X where each X is a 3 digit number from 001 to 255 Note If your instrument is not on a network you do not need to set the IP address Ask your system administrator if the IP address is assigned...

Страница 17: ...r My Profile page The following actions are available from the user profile screen Change a password Create a new user profile Grant administrator rights to selected user profile Administrator only De...

Страница 18: ...nect account Share captured images within a research team or with colleagues in another laboratory location or country Upgrade instrument software automatically without hardware or manual updates Admi...

Страница 19: ...enerate a QR code 1 Download the Instrument Connect Mobile Application on your mobile device a For iPad or iPhone devices download the application from the Apple App Store by searching for Instrument...

Страница 20: ...rom the top navigation strip 4 Select Power Snap Plus from the drop down menu then click Next 5 Enter the linking code generated by the instrument in the text box then click Send Upon successful authe...

Страница 21: ...code from the instrument 1 Open the Notifications screen on the instrument 2 Select Connect to generate a link code and QR code 3 Copy down the link code generated by the instrument or take a picture...

Страница 22: ...ect from the left navigation strip 3 Select Add an Instrument from the top navigation strip 4 Select Power Snap Plus from the Instrument type drop down menu then click Next 5 Enter the linking code ge...

Страница 23: ...lect the username to sign out and connect a different user account 3 Select your username from the list of linked accounts 4 Enter your Connect PIN number If you do not have a PIN number set the PIN n...

Страница 24: ...E Gel SizeSelect II Agarose Gels see page 60 User interface overview Symbol Function Main dial Load protocol Initiate gel run workflow Displays the instrument status when a protocol is run see Touchsc...

Страница 25: ...nes for best result Prepare DNA sample in deionized water or 1X E Gel Sample Loading Buffer Use the indicated amount of DNA per well for single or multiple bands If you are unsure how much to use test...

Страница 26: ...torted or incomplete band separation E Gel DNA Ladder E Gel EX E Gel EX Double Comb E Gel with SYBR Safe E Gel Double Comb with SYBR Safe E Gel 48 with SYBR Safe E Gel 96 with SYBR Safe E Gel Ultra Lo...

Страница 27: ...l empty wells Keep all sample volumes uniform If you do not have enough samples to load all the wells of the gel load an identical volume of deionized water into any empty wells Prepare your samples b...

Страница 28: ...a module 1 Select Set up run to start E Gel protocol selection 2 Select the Category corresponding to the E Gel cassette in the device 3 Select the Type corresponding to the E Gel cassette in the devi...

Страница 29: ...SizeSelect II E Gel 96 with SYBR Safe and E Gel 96 with SYBR Safe agarose gels For optimal viewing dim the ambient lighting in the room or use the E Gel Power Snap Plus Camera for visualization see Vi...

Страница 30: ...otocol duration using the buttons or tap the duration field to open a keyboard to enter a number 4 Select Resume to restart the run Note Do not run the same gel multiple times or extend the gel protoc...

Страница 31: ...gel image Edit adjust capture settings Export image Gallery screen to access Actions screen to Edit Delete or Export images Sort images Capture gel image Edit gel image Export gel image Return to Home...

Страница 32: ...ar of the handhold 2 Lift the camera up off of the base module View gel 1 Select View Gel to access the view gel screen and visualize the bands on the gel 2 Adjust exposure setting if necessary Note T...

Страница 33: ...nction exported or deleted The following additional operations can be performed by selecting an image and selecting Actions View image information Rename files Perform batch rename of files Adjust ima...

Страница 34: ...ibutes to set the attributes that will be used in the file name There are two Custom fields that can be used to add custom text to the file name Note To perform a batch rename select multiple files or...

Страница 35: ...e and inverts color palette Grayscale Converts image into a grayscale Deconvolute image Deconvolution is a process that reconfigures the staggered well format from digital images of 96 well gels into...

Страница 36: ...te 5 Select the layout of the deconvoluted lanes 2 52 4 26 8 13 6 Select Deconvolute Export image Images can be exported from the capture screen or the image gallery The number of images captured in a...

Страница 37: ...me screen 3 Select Thumbnails or List view for navigation Note Use the checkbox to select all images or apply the filter to set parameters for the images to display in the gallery 4 Review the images...

Страница 38: ...proposed method will not damage the equipment Clean the surface of the E Gel Power Snap Plus Electrophoresis Device with a damp cloth Do not use harsh detergents or organic solvents to clean the unit...

Страница 39: ...ime may indicate the need to replace the battery 1 Open the battery compartment on the underside of the E Gel Power Snap Plus Camera 2 Place the battery compartment cover to one side 3 Remove and repl...

Страница 40: ...ally restart Download new firmware 1 Go to thermofisher com from your web browser 2 Click Product Support4Technical Resources4Product Support4Software Patches Updates 3 Select E Gel Power Snap Plus El...

Страница 41: ...ftware to proceed to the Software Update screen 2 Select Settings 4Maintenance Services4Software Update4USB drive The Software Update screen opens 3 Choose the row with the new firmware file from the...

Страница 42: ...Contact your service representative in case of block failure Restore factory settings Administrator profile only Select Restore factory settings to remove all the data and customized settings and rev...

Страница 43: ...n 1X concentration of 10X Sample Loading Buffer Physical gel damage Avoid touching the gel well with the pipette when loading the sample Band distortion caused by air bubbles Avoid introducing bubbles...

Страница 44: ...l Wells damaged during comb removal Remove the gel comb gently without damaging the wells DNA sample cannot be seen Inhibition of visualization by heat Wait 10 15 minutes for gel to cool before visual...

Страница 45: ...and instrument statistics Select EULA to view the End User License Agreement or download it to a USB drive Select Check updates to find out if updates are available for the instrument Recommended ins...

Страница 46: ...p to 25 alphanumeric characters to identify the instrument Note The instrument name cannot have spaces Separate consecutive characters with a hyphen or underscore for example My_Instrument Brightness...

Страница 47: ...e cannot have spaces Separate consecutive characters with a hyphen or underscore for example My_Instrument Date and time Select the Time Zone field to set the time zone Select the Date Format field to...

Страница 48: ...Time field to activate the numeric editor and set the length of time that the instrument can remain idle before it goes to sleep mode Brightness Use the slider to adjust the brightness of the touch s...

Страница 49: ...sequential numbering and order of text to be automatically included in the name of saved files Cloud region Administrator profile only Select the appropriate field to set the cloud region for the ins...

Страница 50: ...and other components See Maintenance on page 38 for instructions on conducting the self verification test Administrator only Select Export Instrument Log to export the instrument logs to a USB memory...

Страница 51: ...ion path 8 Cathode Nucleic acid stains used in E Gel agarose gels SYBR Safe DNA gel stain SYBR Safe DNA gel stain has been specifically developed for reduced mutagenicity making it safer than ethidium...

Страница 52: ...ipality for appropriate disposal in your community Opening E Gel cassettes Electrophoresis must be complete before opening the E Gel cassette Photograph the gel before opening the cassette If you plan...

Страница 53: ...the gel knife into the groove around the edge of the cassette edge then lever the knife up and down to crack the seal 3 Unseal the plate by working around the perimeter of the entire cassette and crac...

Страница 54: ...gel slice to a microcentrifuge tube Cleaning and storage Clean the Gel Knife with mild detergent and water after use and store at room temperature E Gel agarose gel disposal guidelines Discard E Gel E...

Страница 55: ...uidance on disposal of used gels see SYBR Safe DNA Gel Stain SYBR Safe DNA gel stain on page 51 Prepare samples Prepare up to 25 L of sample in 1X Sample Loading Buffer e g use 2 5 L of 10X Sample Loa...

Страница 56: ...ce starting from the right edge 5 Press down on the left side of the cassette to secure it into the device Load samples 1 Fill all wells of both rows with 50 L of deionized water 2 Load 25 L of sample...

Страница 57: ...otocol by pressing Start run The run stops automatically after the programmed time has elapsed Check status 1 Check the gel status by activating the Back light Monitor the gel during the run to avoid...

Страница 58: ...Stop the gel and recover the sample with a pipette Avoid piercing the agarose Some residual DNA will remain visible in the well due to migration into the agarose at the bottom of the well 3 Proceed w...

Страница 59: ...migrate differently DNA fragment size amount and salt content can affect migration rates Table 3 E Gel 1 Kb PLUS express DNA ladder migration pattern Ladder Fragment size DNA amount per 25 L Migration...

Страница 60: ...I gel stain on page 52 Prepare samples Prepare up to 25 L of sample in 1X Sample Loading Buffer e g use 2 5 L of 10X Sample Loading Buffer with 22 5 L total sample 10X Sample Loading Buffer is provide...

Страница 61: ...Device starting from the right edge 5 Press down on the left side of the cassette to secure it into the device Load samples 1 Fill all wells of both rows with 50 L of deionized water 2 Load 25 L of s...

Страница 62: ...essing Start run The run stops automatically after the programmed time has elapsed Check status 1 Check the gel status by activating the Back light Monitor the gel during the run to avoid the target f...

Страница 63: ...the edge of the wells Collect DNA fragment 1 Resume the run and carefully observe as the reference band enters the recovery well IMPORTANT See NGS library size selection reference to determine when to...

Страница 64: ...Gel protocol if the band of interest passes the recovery well see Change to another protocol on page 30 Appendix E E Gel SizeSelect II Agarose Gels Collect DNA fragment E 64 E Gel Power Snap Plus Elec...

Страница 65: ...NA fragment size amount and salt content can affect migration rates Table 4 E Gel sizing DNA ladder migration pattern Ladder Fragment size DNA amount per 25 L Migration time to reference line 1 500 bp...

Страница 66: ...0 base read 390 bp 13 16 min 500 ng 400 bp band is at the middle of the exposed agarose area 50 100 ng 500 bp band is at the top of the exposed agarose area 200 base read 330 bp 12 14 min 500 ng 350 b...

Страница 67: ...area 150 base read 220 bp 11 14 5 min 500 ng 250 bp band is at the middle of the exposed agarose area 50 100 ng 250 bp band is at the middle of the exposed agarose area Quantitation of isolated DNA Re...

Страница 68: ...e 50 000 hours LED specification Array of 39 high power LEDs emitting at 465 10 nm Specification AC DC power adapter 1 Rated Voltage Input 100 240 VAC Rated Current Input 1 3 A Rated Frequency Input 5...

Страница 69: ...een LCD display 115 2 mm 86 4 mm Camera type color CMOS Gel image resolution 1600 1944 3MP 8 bits Dynamic range 68dB Image output tif Grayscale and jpg Color Lens f number 2 8 Appendix F Specification...

Страница 70: ...Target fragment isolation in cloning workflow Low range fragment library size selection in NGS workflow High range fragment library size selection No rows 2 rows 1 loading row and 1 recovery row 2 ro...

Страница 71: ...nes Loading volume 20 L 20 L 20 L 20 L 15 L 20 L Stain SYBR Gold II SYBR Safe SYBR Safe Detection sensitivity 0 5 ng band 5 ng band 5 ng band Agarose 1 2 4 1 2 1 2 4 1 2 1 2 4 1 2 Separation range 1 1...

Страница 72: ...44888 2 10 gels A42346 20 gels A44889 50 gels A44890 E Gel Agarose Gels with SYBR Safe 1 10 gels A42100 20 gels A45202 50 gels A45203 2 10 gels A42135 20 gels A45204 50 gels A45205 4 10 gels A42136 20...

Страница 73: ...G720841 2 8 gels G720802 32 gels G720842 E Gel NGS Agarose Gels 0 8 10 gels A25798 E Gel CloneWell II Agarose Gels 0 8 10 gels G661818 E Gel SizeSelect II Agarose Gels 2 10 gels G661012 E PAGE Protei...

Страница 74: ...ge DNA Marker E Gel Low Range Quantitati ve DNA Ladder E Gel Ultra Low Range DNA Ladder E Gel Sizing DNA Ladder Gel Type Agarose Cat No 10488090 Cat No 10488091 Cat No 10488099 Cat No 12352019 Cat No...

Страница 75: ...0488100 E Gel Low Range Quantitative DNA Ladder 17 5 ng L 1 mL 100 apps 12373031 E Gel Ultra Low Range DNA Ladder 25 ng L 2 1 mL 100 apps 10488096 E Gel 96 High Range DNA Marker 5 ng L 2 1 mL 100 apps...

Страница 76: ...required skills be allowed to use the product Keep the basic safety instructions and the product documentation in a safe place and pass them on to the subsequent users Applicable local or national saf...

Страница 77: ...not place the product in an area where its ventilation system is obstructed Lack of proper ventilation may result in electric shock fire and or serious personal injury or death The product is for ind...

Страница 78: ...Electrical Equipment for Measurement Control and Laboratory Use Part l General Requirements Instruments bearing the UL symbol are certified by Underwriters Laboratories to be in conformance with the a...

Страница 79: ...d une mani re non sp cifi e par le fabricant la protection offerte par l appareil pourrait s en trouver d t rior e Ce produit est conforme UL 61010 1 CAN CSA C22 2 No 61010 1 Exigences de s curit pou...

Страница 80: ...he actual current draw of the instrument is expected to be approximately 72 W 245 67 Btu h Vibration Ensure that the instrument is not adjacent to strong vibration sources such as a centrifuge pump or...

Страница 81: ...ire two conductors and ground power cable and plug Use this power cable with a properly grounded electrical outlet to avoid electrical shock If extension cords or connector strips are implemented they...

Страница 82: ...or injuries Prior to cleaning the product disconnect it completely from the power supply Use a soft non linting cloth to clean the product Never use chemical cleaning agents such as alcohol acetone or...

Страница 83: ...cedures as recommended in the SDS Handle chemical wastes in a fume hood Ensure use of primary and secondary waste containers A primary waste container holds the immediate waste A secondary container c...

Страница 84: ...should be trained according to applicable regulatory and company institution requirements before working with potentially biohazardous materials Follow all applicable local state provincial and or na...

Страница 85: ...cols Certificates of Analysis Safety Data Sheets SDSs also known as MSDSs Note For SDSs for reagents and chemicals from other manufacturers contact the manufacturer Limited product warranty Life Techn...

Страница 86: ...r Snap Plus Electrophoresis System UG v1 GUID B3EEBB8A E81D 45C4 8442 4EAB97C0873E 2021 10 21 18 35 43 en 22 16 57 068 01 00 thermofisher com support thermofisher com askaquestion thermofisher com 5 A...

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