Prepare wells
1.
Open the filter lid of the E
‑
Gel
™
Power Snap Plus Electrophoresis Device and activate the Back
light.
The transilluminator turns off automatically when the filter lid is opened. Press
Back light
to
re-activate the blue light transilluminator.
2.
Carefully remove all liquid from the recovery wells.
3.
Load 50 µL of nuclease-free water to all recovery wells. Do not allow water to spill over the edge of
the wells.
Collect DNA fragment
1.
Resume the run
and carefully observe as the reference band enters the recovery well.
IMPORTANT!
See NGS library size selection reference to determine when to collect samples of
specific target library length.
2.
Stop the gel and recover the sample with a pipette. Avoid piercing the agarose.
Some residual DNA will remain visible in the well due to migration into the agarose at the bottom of
the well.
3.
Proceed with downstream NGS workflow.
Appendix E
E
‑
Gel
™
SizeSelect
™
II Agarose Gels
Prepare wells
E
E
‑
Gel
™
Power Snap Plus Electrophoresis System User Guide
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