Determine whether an amplification curve is acceptable based
on the following:
Clear knee points, particularly distinctive low-concentration sample exponential
phase. Parallel amplification curve as a whole, flat baseline without rise, and
distinctive low-concentration sample exponential phase.
Slope of exponential phase in direct proportion to amplification efficiency - the
more slope, the higher amplification efficiency.
Standard flat and straight baseline or with slight descent, but without obvious
rise trend.
Instructions on Amplification Curve
Test Considerations
●
Rules and regulations issued by the authorities shall be complied with by the
laboratory.
●
Fluorescent material-free disposable gloves, disposable dedicated centrifugal
tube, self-removing pipette and suction head with filter element shall be used.
●
Prepare and handle reaction fluid in such way to avoid generation of air bubble;
check whether reaction tube is sealed tightly prior to startup to prevent leakage
of fluorescent material and thereby pollution to the instrument.
●
During loading sample soak the sample entirely into the reaction fluid and
prevent attachment of any sample onto the tube wall; seal the tube after loading.
●
Take the reaction tube out immediately after completion of amplification, and
seal it in dedicated plastic bag and keep the bag at specified location for
approval and treatment.
