Specific Test Procedure
Locate reagent
First align the mark on the knob with point unlock,
and then slide heated lid to put sample reagent into
Hole 96 and then close the lid; rotate the knob
clockwise to a proper position to run the program for
testing. It is recommended to align mark on the knob
with the point LOCK.
Features of Real-Time Quantitative PCR
Specificity: Quantitative PCR features both primer and probe, so is more special than
conventional PCR.
Sensitivity: Sensitivity of fluorescence PCR is up to 10² copies/ml, and the range of linearity in
logarithmic phase analysis is 0~10
11
copies/ml. Generally, the number of pathogens in a
clinical specimen is 0~10
10
/copy, within which quantitative PCR will dose accurately and the
specimen need not be diluted.
Repeatability: Quantitative PCR presents a stable result, since the threshold value is set in
exponential amplification phase, during which, concentration of the reaction constituents is
relative stable, free of side effect, and the logarithm of CT and fluorescence signal are in a
linear relationship. Compared to Endpoint Method CT value is more stable and more
accurately reflects copies of initial template.
Safety: Without post-PCR operation steps, it reduces risk of pollution by the products.
