CT Value
Means the cycles which fluorescence signals in each PCR reaction tube undergo to
reach the Threshold value as set. According to a research, CT value of each template
is in a linear relation to the logarithm of initial copies of this template. The more
initial copies, the fewer CT value, vice versa. A standard curve can be created by
using a standard product in known initial copies, where the abscissa represents
logarithm of initial copies, and the ordinate indicates CT value. Therefore, initial
copies for an unknown sample can be calculated from the standard curve only by
determining CT value of the sample.
In this table you can view CT
and TM values of a certain curve
and other result information.
Fluorescence Threshold Setting
Generally fluorescence signals in the first 15 cycles in PCR reaction are
used as fluorescence background signals. Fluorescence Threshold value is
10 times of standard deviation of fluorescence signals in 3~15 cycles, and
set in the exponential phase of PCR amplification.
