28
X-Cite
®
Optical Power Measurement System User Guide
035-00390R rev. 3
9.4
Calibrating X-Cite
®
exacte
with the XP750
9.4.1
Leave the X-Cite
®
exacte
light guide connected to your microscope as you would
during normal operation. Keep the X-Cite
®
exacte
positioned as you would
during normal operation.
9.4.2
Place the XP750 onto your microscope as described in
section 4
, and connect it
to an XR2100.
9.4.3
Connect the XR2100 to the X-Cite
®
exacte
using the supplied Stereo Connection
Cable. Refer to
section 4
9.4.4
IMPORTANT: The XP750 will calibrate X-Cite
®
exacte
using data from only one
wavelength setting. It is important to select the wavelength most applicable to
your application prior to calibrating the X-Cite
®
exacte
using the system. The
ideal wavelength setting is dependant on your application and typically
corresponds to the excitation maxima (peak) of the fluorophore. Please refer to
section 4.2.9
section 4.3.8
for setting the wavelength being measured by the
system.
9.4.5
Press the X-Cite
®
CAL
button on the XR2100 to perform an XP750 calibration.
Note:
X-Cite
®
exacte
internal software version 2.0 or higher is required to accurately display
the lower power measurements from XP750 calibration. Software version is displayed
during X-Cite
®
exacte
start-up sequence. Contact Lumen Dynamics if a software
upgrade is required.