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LS720 Microscope Manual 2021
13.
Click on the Focus Settings button to open its dialog box.
There are 4 options to set up focus in a Protocol:
a.
Manual Focus. Uncheck both Use Auto-Focus and Use Z-
Map. Under Manual Focus Control, click the up and down
arrows to determine the desired focus level. This will be
the focus level for all locations in the Protocol. It will
need to be updated for each running of the Protocol.
b.
Autofocus. Check Use Auto-Focus and uncheck Use Z-Map.
Under Autofocus Parameters, click on Use Manual Settings to
load focus settings previously determined in Autofocus Setup launched from Manual XYZ.
c.
Z-Map. Uncheck Use Auto-Focus and check Use Z-Map. Follow steps described above to
create a Z-Map for each location to be imaged in the Protocol. To use an existing Z- Map, select
the file under Z-Map File Selection.
d.
Autofocus plus Z-Map. Check both Use Auto-Focus and Use Z-Map. Set Autofocus Parameters
as described above in Step b, this Section. Follow steps described below in Section II.L. to
create a Z- Map for each location to be imaged in Protocol. To use an existing Z-Map, select the
file under Z- Map File Selection.
e. Under Select Focusing LED Channel(s), check the channels desired for autofocusing.
Currently, channels are acquired in a fixed sequence of BF, Blue, Green, Red. Any selected
autofocus will also occur in this sequence. If using autofocus, you must select the first LED
channel to be imaged as an autofocusing channel per the listed order above. The subsequent
channels in your protocol channel sequence are optional for autofocusing. If the subsequent
channels are not chosen, the focus will stay at the level determined for the first channel in
the sequence. For example, if you are snapping brightfield and red channel images, select at
least the brightfield channel for focus. Autofocus will occur in the brightfield channel and
then remain at that level to capture the red channel image. If you want the red channel to
also be focusing, select both the brightfield and red channels.
