ERBA ELite 5, Руководство пользователя

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ELite 5, User Manual
Page 73 of 159
WHOLE BLOOD
SAMPLE
WHOLE BLOOD
SAMPLE
MIX DILUTION
IMPEDANCE
MEASUREMENT
+
LIGHT
ABSORBEANCE
DILUENT
LYSE
IMPEDANCE
MEASUREMENT
DILUENT
DILUENT
TEMPERATURE
CONTROLLED
INCUBATION
LYSE
LYSE2
OPTICAL
MEASUREMENT
OPTICAL
MEASUREMENT
TOTAL WBC, HGB RBC, PLT
4DIFF
BASO
Figure 52. ‘ELite 5’ Measurement Process
When you present the sample tube and press the Start button, the ‘ELite 5’ rotates the
sample rotor and takes the sample tube inside the analyzer. The piercing needle pierces the
sample tube cap (if present) and aspirates approximately 110 µL of primary blood sample.
Blood sensors ensure that the primary blood sample is continuous and free of major bubbles
as it is moved to the shear valve. The shear valve separates the primary sample into further
samples of exact volumes of blood that are distributed to provide dilutions for the RBC, WBC,
HGB and 5 Diff population measurements. Lysing reagents are used to eliminate RBCs for
dilutions that measure WBCs.
RBC/PLT and WBC are measured with impedance technology through 70 and 80 µm
apertures respectively. Two portions of the sample are used for determining a five
subpopulation differential of WBC in two independent 4DIFF and BASO measurements.
Sample results are displayed on the screen as the process is nearing completion. Results
are stored in the internal database and transmitted to an external LIS if configured to do so.
The ‘ELite 5’ performs a cleaning rinse of the internal pneumatic components and prepares
to measure the next sample.