Intended use
qTOWER³ / qTOWER³ G
6
2
Intended use
NOTICE
The device is intended for
general laboratory use
. Its use is limited to applications out-
side of in-vitro diagnostics (research use only).
The device may only be used for the applications described in these operating instruc-
tions.
The manufacturer does not accept liability for any other use.
The device is a thermocycler which is licensed for real-time PCR experiments that am-
plify DNA via polymerase chain reaction (PCR) while using fluorescence spectroscopy for
the highly sensitive detection of target sequences. The signal from the fluorescent dyes
excited by a light source correlates quantitatively with the amount of PCR product and
may be shown in real-time.
The integrated detector allows measurement of the sample flourescence in up to six
spectral channels during PCR, providing verification of multiple target sequences in a
single PCR reaction. The filters used on the color or FRET modules are precisely tuned to
the properties of the fluorescent dye most often used, allowing sensitive and selective
detection of fluorescent PCR products.
Analytik Jena offers a series of color or FRET modules, with up to six being able to be si-
multaneously installed in the device.
The replacement and retrofit of color or FRET modules is easy.
The device is an open platform for real-time PCR and supports both intercalating dyes as
well as individual sensors and kits from various manufacturers. The device can be used
in various applications, such as expression analyses, genotyping and the detection of
pathogens.
The device is fully controlled from the PC using the qPCRsoft software. The software
provides the following functions:
¡
Device control and monitoring
¡
Context-sensitive help functions
¡
Design of real-time PCR experiments and their evaluation
¡
Storage of methods (templates) and measuring results (projects)
¡
User management
¡
Planning and evaluation of
– Absolute quantifications
– Relative quantifications
– ΔΔCt analyses
– DNA melting curves
– Genotyping
– End point analyses
¡
Results export to MS EXCEL or as CSV file
¡
Results printout
¡
Results export to extended programs for the analysis of real-time PCR data (e.g.
GenEx, qBASE)
A detailed description of the software can be found in the software manual.
We recommend the following scientific publication for an intensive introduction into the
real-time PCR analysis techniques and applications: