qTOWER³ / qTOWER³ G
Operation
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When using few well strips, place one strip on each side of the sample block.
Figure 11Positions of additional 8-well strips when measuring with few samples
Start a real-time PCR analysis as follows:
}
Pipette the PCR samples into the sample vessels. Close the sample vessels.
NOTICE! Micro titer plates must be sealed with optically transparent adhesive foil
(sealing foil). The optical transparency of the foils affects the fluorescence signal di-
rectly. For this reason, only use clear adhesive foil such as that provided for real-time
PCR. 0.2 mL individual tubes and 8-well strips must be sealed with suitable corre-
sponding optical lids.
}
Prepare a real-time PCR project with complete information on the PCR run, floures-
cence measurement and sample layout of the PCR plate.
Figure 12Position A1 on the sample
block
}
Open the lid. To do so, press in the red handle on the front until the lock
clicks open. Fold back the upper part of the device.
}
Place the samples. Observe the information on placing samples when
measuring few samples when doing this. When using PCR plates, place
these on the thermal block so that well A1 is on the left-hand side (arrow
in the illustration below). This position corresponds to the well allocation
in the software.
}
Close the lid. To do this, fold the lid forward and press it down with the
handle until the lock engages with a click.
}
Start the PCR run in the software.
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The PCR run begins and analysis starts.