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Biometra TRIO Creating, editing and saving programs
Version 03_19 47
7.6.4 Adjust ramp rate
The average ramp rate can be adjusted for each step. The ramp rate adjustment can
be useful if PCR programs are transferred from slower Thermal Cyclers the Biometra
TRIO or for special PCR applications like for example telomerase PCR. The average
ramp rate specifies the speed at which the selected step is reached with. To adjust the
ramp rate press the [
RAMPE RATE
] button.
R
AMP
R
ATE
( Δ R)
Then enter a value between 0.1°C/s and max. in tenths of degree Celsius.
It is also possible that the entered ramp rate is applied to all steps in the PCR program.
For this the following checkbox to the right of the input field for the ramp rate must be
activated.
Note : The maximum average rate depends on the sample block installed in the Bi-
ometra TRIO Thermal Cycler. If the ramp rate has been adjusted, the small symbol
will be shown at the corresponding step in the graphical programming mode.
7.6.5 Set Temperature Optimisation Step (TOS)
For optimisation of new PCR protocols very often gradient enabled Thermocyclers are
used. The Biometra TRIO offers no gradient function but by the temperature optimisa-
tion step (TOS) function programs making use of the three blocks and delivering dif-
ferent annealing temperatures at the defined step can be created.
Sample replicates are distributed across the sample blocks and are tested for the tem-
perature giving the optimal result. To program a Temperature Optimisation Step press
the following button.
T
EMPERATURE
O
PTIMISATION
S
TEP
(TOS)
Enter the desired annealing temperature (A
NNEALING
T
EMP
.) and define a temperature
increment (I
NCREMENT
) between the sample blocks. The effective temperatures for all
sample blocks are displayed (see Figure 23).
Note : The annealing temperature is set for sample block 2 and the increment defines
the temperatures for sample block 1 and 3. When setting a positive increment the
temperature in sample block 1 is the lowest and the temperature in sample block 3 is
the highest. Use the minus button on the keypad to enter a negative increment. When
a negative value is used as increment the temperature in sample block 1 is the lowest
and the temperature in sample block 3 is the highest.