Biometra TRIO
Creating, editing and saving programs
Version 03_19
45
Figure 22 Biometra TRIO edit step screen
Note
: To program a step at least the temperature and the hold time must be defined.
To set the temperature touch the corresponding input box and enter a value between
3.0°C and 99.0°C. Use the Tab button or touch the corresponding input box to activate
the next input box and enter the time in h:mm:ss.
The Biometra TRIO software offers to program several steps subsequently. Use the
cursor buttons to navigate between the steps..
P
REVIOUS STEP
N
EXT STEP
The currently activated step is high-lighted in the program overview table (see Figure
22).
Note
: By navigating between steps it is not necessary to confirm the settings for each
single step and to leave the edit step screen but you can fill in the parameters for sev-
eral steps and confirm the settings after all steps are programmed.
7.6.1
Set cycles
A typical PCR program consists of repeated steps for denaturation, annealing and ex-
tension. For the repetition of steps the Biometra TRIO software offers to program
loops. For a cycle two parameters have to be set:
1.
The step number to go back to (G
OTO
)
2.
The number of repeats (C
YCLES
)
To program a loop select the
LAST STEP
of the cycle and enter the step number the pro-
gram shall go back to in the input box G
OTO
(see Figure 22). To define the number of
repeats, enter a number in the input box C
YCLES
.
For example to program the cycles for the following protocol select step 4, enter 2 in
the input box G
OTO
and enter 35
in the input box C
YCLES
.