Creating, editing and saving programs
Biometra TRIO
46
Version 03_19
Figure 23 PCR program with loop
In this example the instrument first executes step 1 to step 4, then repeats step 2 to
step 4 for 34 times, performs the final extension step at 72°C and holds the tempera-
ture indefinitely at 16°C.
Note
: The Biometra TRIO allows to set maximal two interlaced loops. It is possible to
program a loop within a loop but it is not allowed to set loop in loop in a loop.
7.6.2
Set temperature increment
For some applications, like for example touch down PCR, temperature increments are
used. The temperature is increased or decreased with each cycle by the set value. To
program a temperature increment press the following button (see Figure 22) and fill
in a value b20°C and -20°C in the corresponding input box.
T
EMPERATURE
I
NCREMENT
(
Δ
T)
Note
: Temperature increments only become active in repeated steps (cycles). If a tem-
perature increment has been programmed, the small symbol
will be shown at the
corresponding step in the graphical programming mode.
7.6.3
Set time increment
For some applications, like for example long range PCR, time increments are used. The
time is increased with each cycle by the set value. To program a time increment press
the following button (see Figure 22) and fill in a value between 0s and 240s in the
corresponding input box.
T
IME
I
NCREMENT
(
Δ
T
)
Note
: Time increments only become active in repeated steps (cycles). If a time incre-
ment has been programmed, the small symbol
will be shown at the corresponding
step in the graphical programming mode.