Creating, editing and saving programs
Biometra TAdvanced
54
Version 02_20
Fig. 32 Standard gradient programming screen (Biometra TAdvanced Mono)
For Biometra TAdvanced twinblock models
Note
: To be able to use the gradient function, the sample block needs to be gradient
enabled. For Biometra TAdvanced Twin 48 G this is the left block.
The gradient function is most often used to optimize new primer pairs. A temperature
gradient is established across the sample block in the annealing step so that there are
different temperatures from column to column:
Fig. 33 Temperature gradient on a 48 well sample block
In a specific column all wells have the same temperature but the temperature differs
from column to column. The temperature gradient can be applied in both directions;
the highest temperature can be either in column 1 or column 8.
Sample replicates are distributed across the columns of the sample block and tested for
the temperature giving the optimal result. To program a gradient step press the
following key (see Fig. 28)
G
RADIENT