Biometra TAdvanced
Creating, editing and saving programs
Version 02_20
51
Fig. 29 PCR program with a loop
In this example, the device first executes steps 1 to 4, then repeats step 2 to step 4 for
34 times, performs the final extension at 72°C and holds the temperature indefinitely
at 16°C in the last step.
For some applications, for example touch down PCR, temperature increments are
used. The target temperature is increased or decreased in each cycle by the set value.
To program a temperature increment, touch the [
T] button (see Fig. 28) and
enter a value b20°C and -20°C in the corresponding input field.
T
EMPERATURE INCREMENT
(
T)
Note
: Programmed temperature increments only become active if they are in loops. If
a temperature increment has been programmed, the small symbol
will be shown
at the corresponding step in the graphical programming mode.
For some applications, for example long range PCR, time increments are used. The
time is increased in each cycle by the set value.
To program a time increment, touch the
t button (see Fig. 28) and enter a value
between 0 s and 240 s in the corresponding input field.
T
IME INCREMENT
(
t)
Note
: Time increments only become active if they are in loops. If a time increment has
been programmed, the small symbol
will be shown at the corresponding step in
the graphical programming mode.
Programming a
temperature increment
Programming a time
increment