Fault removal
Biometra TAdvanced
118
Version 02_20
14.7
Recommendations for the use of 384 well sample blocks
Use the recommended 384 well plates and sealing materials
To prevent sample evaporation by insufficient sealing use 384 well plates and sealing
materials tested and recommended by Biometra. A list of plates and sealing is
available at http://analytik-jena.com/.
Reduce the temperature of the heated lid
Due to the fact that the heated lid is very close to the reaction volume with a 384 well
plate compared to 0.2 ml vessels the lid temperature can have a significant impact on
the effective incubation temperature. For reaction volumes less than 10
μl the lid
temperature is recommended to be set at 95 °C. When using a reaction volume of 10
μl
or more, set lid temperature at 85 °C. Even at these lowered lid temperatures possible
condensation will not occur as problem due to a mixture of physical factors like dew
point, salt concentration, temperature difference, air pressure within the tube and
reaction volume. It is strongly recommend to reduce the lid temperature according to
the reaction volume. Otherwise, at conventional lid temperature settings, sample
volumes or amplification yields might be reduced.