Biometra TAdvanced
Adaptation of programs
Version 02_20
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Adaptation of programs
If programs are exchanged between different thermal cycler models or the sample
block is exchanged, programs might need to be adapted to the installed sample block
type prior to the start or during editing. The following table provides an overview of
possible program adaptations:
Cause
Example
Adaptation
The maximum gradient
range is exceeded.
A program for the 96 well silver block
with a programmed gradient range of
>30°C is to be started on a 96 well
aluminum block and exceeds the
block's maximum permitted gradient
range.
The temperature range of the gradient will be
reduced based on the average temperature of the
maximum permitted range. If, for example, the
average temperature of the programmed gradient
is 60°C and the gradient range is ± 20°C, the
gradient will be reduced to 60°C ± 15°C.
A program with a gradient
step is transferred to a
non-gradient-enabled
device.
The program with the gradient step is
started on a sample block without
gradient function.
The gradient will be deleted and the average
temperature or annealing temperature will be used
for this step. If the average temperature or
annealing temperature of the programmed
gradient is, for example, 60°C, this value will be
used for the step.
The gradient is outside the
permitted limit.
A program for the 96 well silver block
with a gradient temperature below
20°C is to be started on a 96 well
aluminum block and falls below the
minimum permitted temperature.
The lower temperature is increased to the
minimum permitted value. If, for example, a
gradient is programmed from 5°C to 25°C, the
gradient is changed to 20°C to 25°C.
A program with a
temperature optimization
step (TOS) is transferred to
a device that does not
offer this function.
A TOS program is transferred from
the Biometra TRIO to another cycler
type.
The average value of the temperatures
programmed in the TOS step for the left and right
sample block is used.
The heating and cooling
rate is exceeded.
A program for a 96 well silver block is
to be started on a 96 aluminum block.
The heating and cooling rate will be adjusted to the
maximum possible value for the installed sample
block type. If the programmed heating and cooling
rate is, for example, 8°C/s, it will be reduced for the
aluminum block to 6°C/s.
The heating and cooling
rate is not reached.
A program for a 96 well aluminum
block is to be started on a 96 silver
block.
The heating and cooling rate will be increased to
the maximum possible value for the installed
sample block type. If the programmed heating and
cooling rate is, for example, 6°C/s, it will be
increased for the silver block to 8°C/s.
User-defined heating and
cooling rate.
The heating and cooling rate is lower
than the maximum value for a
program step.
The user-defined value is maintained and not
adjusted.
Note
: If programs need to be adapted to the installed sample block, a message is
always displayed prior to the start or during editing. The user can confirm or reject the
adaptations. If a necessary adaptation is rejected during the attempt to start a
program, the program cannot be started by the device.