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4:

Operating Instructions

57

To Save an Image with Reference

1

Place the sample on the MicroChemi Sample Tray.

2

Capture a chemiluminescent image.

3

Click the Save Image with Reference

icon on the GelCapture tool bar.

4

A pop up message will be displayed asking you to verify the illumination is turned on, prior to
taking the reference image.

5

The save dialog box will be displayed.

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Содержание MicroChemi

Страница 1: ...Welcome to the MicroChemi System...

Страница 2: ...ext and graphics are for the purpose of illustration and reference only The specifications on which they are based are subject to change without notice Information in this user guide is subject to cha...

Страница 3: ...Box 11 Hardware Setup 12 Software Installation 12 Installing GelCapture from CD Windows XP Pro 13 Installing GelCapture from CD Windows 7 Professional 17 Installing the Camera Drivers Windows XP Pro...

Страница 4: ...d Image Series 48 Saving Features 55 To Save an Image 55 Saving Options 56 Save Image with Reference 56 Open an Image 62 Imaging Tools 63 Image Statistics 63 Inverted Image 64 Pseudo color 65 Overexpo...

Страница 5: ...the system s different features and procedures Everything you need to know about your MicroChemi system is included in this manual The highlighted chapter below contains information you may need to r...

Страница 6: ...g the perfect solution for high quality imaging results System Overview The gel sample is placed on the MicroChemi Drawer Tray and the GelCapture image detection software detects and saves the specime...

Страница 7: ...logy providing clear images with minimum background noise Intuitive software and hardware operation Detailed sample viewing made available by real time high magnification Click of a button software in...

Страница 8: ...er Tray is uniquely designed to facilitate sample image analysis The samples are placed on the Drawer Tray which is modeled with a bordered niche to ensure the sample solvents will not to leak out ont...

Страница 9: ...he MicroChemi unit thereby giving you complete control of configuration capturing and saving sample images on your PC for analysis The friendly GUI lets you perform common actions with a single click...

Страница 10: ...Exposure Time Sets the exposure time Capture Type Select either a Single Image or an Image Series for capturing Binning Set the camera Resolution and Sensitivity Camera Ready Indicator The indicator...

Страница 11: ...can then paste the image in a number of Windows applications Invert Image Display an inverse version of the image Pseudo color Show the image in Pseudo colors Show Hide Over Exposure Show overexposed...

Страница 12: ...aximum ambient humidity 80 Do not store the system in direct sunlight or in the direct flow of the air conditioner Do not clean the system with harmful solvents Only use a soft cloth dampened with wat...

Страница 13: ...he following software from the CDs provided inside the MicroChemi package GelCapture Windows XP Pro page 13 Windows 7 Professional page 17 Camera drivers Windows XP Pro page 23 Windows 7 Professional...

Страница 14: ...t feedback and input it is therefore advised to closely oversee the installation process Note Before starting installation verify that you are logged in as Local Administrator 1 Insert the installatio...

Страница 15: ...Setup and Installation 14 2 Click Next to install to the default location or click Browse to select a different location 3 From the drop down menu select the appropriate system to install and click Ne...

Страница 16: ...3 Setup and Installation 15 4 Mark the checkbox if you wish to create a desktop icon and click Next...

Страница 17: ...stallation 16 5 Verify that the installation information is correct and click Install After you click Install the installation progress will be displayed 6 Once installation is completed click Finish...

Страница 18: ...the installation file GelCapture exe and select Run as administrator The drop down list will close after the selection is made 2 In the CD again locate the GelCapture exe file and double click it to s...

Страница 19: ...3 Setup and Installation 18 4 Click Next 5 Click Next to install to the default location or click Browse to select a different location...

Страница 20: ...3 Setup and Installation 19 6 Click the combo box and select the MicroChemi component from the list...

Страница 21: ...3 Setup and Installation 20 7 Click Next to continue installation 8 In the Select Additional Tasks window choose if you want to create a GelCapture desktop icon Click Next to continue the installation...

Страница 22: ...3 Setup and Installation 21 9 Verify that the installation information is correct and click Install 10 The installation progress window will be displayed...

Страница 23: ...Finish to exit Setup 12 The GelCapture image capturing software for Windows 7 Professional is now installed on your computer Note To activate GelCapture in Windows 7 Professional right click the GelCa...

Страница 24: ...Installing the Camera Drivers Windows XP Pro To Install the Camera Drivers Windows XP Pro 1 Turn the MicroChemi unit on Windows XP Pro automatically displays the Found New Hardware Wizard Choose No n...

Страница 25: ...3 Setup and Installation 24 2 While the Wizard installs the drivers the following window will be displayed 3 If the Windows Logo testing window is displayed choose Continue Anyway...

Страница 26: ...and Installation 25 4 Once the Wizard completes the drivers installation click Finish 5 Double click the desktop GelCapture icon to open the program GelCapture will now recognize the camera and its d...

Страница 27: ...rs are not installed automatically please follow the following installation instructions Before installing the camera drivers you must first install GelCapture software for Windows 7 Professional on y...

Страница 28: ...3 Setup and Installation 27 2 Select Small icons from the Control Panel View by list 3 Double click the System icon System icon Small icons...

Страница 29: ...ect Device Manager from the left side of the window and click Continue in the next window 5 Turn your MicroChemi unit ON The Device manager should recognize the newly connected device and alert you De...

Страница 30: ...3 Setup and Installation 29 6 Under Other devices right click MicroLine ML2020 and select Update Driver Software 7 In the Update Driver Software window select Browse my computer for driver software...

Страница 31: ...3 Setup and Installation 30 8 Select Let me pick from a list of device drivers on my computer 9 Select the icon for the MicroChemi camera driver and click Next Icon selection...

Страница 32: ...3 Setup and Installation 31 10 Choose DNR Camera and click Have Disk 11 In the Install From Disk window click Browse...

Страница 33: ...3 Setup and Installation 32 12 Browse to the following location My computer Local Disc C Program Files DNR GelCapture drivers FLI and click Open 13 Choose fliusb driver and click Open...

Страница 34: ...3 Setup and Installation 33 14 Click OK 15 Choose DNR Camera and click Next...

Страница 35: ...he driver update the successful update window will be displayed Click Close 18 Close the Device Manager and Control Panel windows The MicroChemi camera drivers are now installed and the camera is read...

Страница 36: ...y as part of preventive maintenance procedures You can either calibrate the camera immediately after set up and installation or at any other time when you open GelCapture Whenever you open GelCapture...

Страница 37: ...calibration files 4 After the camera calibration files have been imported successfully a message box will inform you that calibration files have been created successfully 5 When you choose option 2 2...

Страница 38: ...utes box will automatically be marked Click Acquire to continue 7 The CCD Calibration Progress window appears and displays the Dark images and Bias images Calibration progress while the system creates...

Страница 39: ...rm Camera Calibration you can activate it at any time from GelCapture s menu bar The steps to take are identical to the calibration steps described above except for the manner of activating the proces...

Страница 40: ...ollowing workflow allows you to easily capture and save an image Turn the MicroChemi Unit ON Turn the MicroChemi Camera ON Insert a sample Open GelCapture Preview the image Capture an image Save the i...

Страница 41: ...erature ranges between 50o C and 60o C When the temperature is higher than this range the temperature indicator is red When the camera reaches operating temperature the indicator turns green Inserting...

Страница 42: ...orrectly on the Drawer Tray To Preview an Image 1 Turn the White Light WL button on 2 Click the Preview button In GelCapture s main screen A message will be displayed reminding you to turn the illumin...

Страница 43: ...The following message will be displayed in GelCapture s main screen asking you to turn the illumination OFF Note Before you click the start button to capture the image you must first set the Exposure...

Страница 44: ...Exposure Time Exposure time defines the period of time during which the camera collects photons of light that emit from the sample Therefore increased exposure time means more time for light to reach...

Страница 45: ...window for entering a value will be displayed Insert the preferred time value to set the Exposure time accordingly Automatic Exposure Time When you only roughly know the appropriate exposure time for...

Страница 46: ...the Auto Exposure results higher binning will result in lower exposure time 4 Once evaluation is completed a dialog box will be displayed asking what type of information is required a Quality choose t...

Страница 47: ...a larger pixel you can create a higher ratio of Signal Noise for example when using Binning to create an area of 4 pixels 2X2 array This big new pixel which is created from 4 normal sized pixels incre...

Страница 48: ...used When you select Standard Resolution the image will be detected in very high resolution and normal camera sensitivity Other options such as High Sensitivity Super Binning etc will increase the cam...

Страница 49: ...he Software Integration or Hardware Integration option depending on your project s requirements See the explanation on Integration types on page 49 Capturing a Single Image 1 From the Capture Type dro...

Страница 50: ...and combined with image 1 to create image 2 The image is captured for two minutes Image 3 3 minute exposure time The last image composite image 2 is capture for one minute and combined with image 2 t...

Страница 51: ...GelCapture s main screen 3 The Image Series settings will be displayed on the left side of the screen 4 Use the Number of Images slider to select the required number of images 2 20 The Process Summary...

Страница 52: ...k OK you can change the Delay value of a specific image by left clicking the cell in the Delay column from the Process Summary table which will open the Set Delay time window 8 Select the type of disp...

Страница 53: ...ocess you can click the Acquire button to start the process from the beginning using the values you already set This will overwrite the images that were already acquired in the aborted process unless...

Страница 54: ...bove When the capturing process is complete the Average result of the Image Series will be displayed in GelCapture s main view b Click Acquire if you want to recapture the series or click the Return b...

Страница 55: ...ng the Abort button and only the images that were captured up to that point will be displayed in the main view 9 After aborting the process you can click the Acquire button to start the process from t...

Страница 56: ...n of two separate images Read the following section to learn more about the saving options To Save an Image After setting your preferences Exposure Capture Type Binning continue to capture the image a...

Страница 57: ...excitation source and have visible standard which is detected by white light illumination With the unique Save Image with Reference feature you can save one image of a multi dyed sample thus making yo...

Страница 58: ...roChemi Sample Tray 2 Capture a chemiluminescent image 3 Click the Save Image with Reference icon on the GelCapture tool bar 4 A pop up message will be displayed asking you to verify the illumination...

Страница 59: ...7 The sample which is illuminated with WL is displayed in the Save Image with Reference viewer If the image is very dark or very bright you may need to use the Dimmer dial on the front of the unit to...

Страница 60: ...he standard image If the standard can hardly be seen in the chemiluminescent image adjust the Threshold slider and click Copy or Copy Inverted again Threshold helps you reach the ultimate dynamic rang...

Страница 61: ...ample standard which is stained with a visible dye 10 When you have finished copying all the required standards click Done If you want to start the standard copy process from the beginning click Rever...

Страница 62: ...add more standards If you click Yes the combined image will be displayed in the main viewing area and you can proceed If you will click No the last screen will be displayed and you will need to select...

Страница 63: ...turn the illumination OFF will be displayed Open an Image To Open an Image File 1 In GelCapture click the Open image icon 2 Select the image to open from the DNR File Open dialog window 3 Click Open T...

Страница 64: ...GelCapture the changes you made and saved are displayed For example if you invert an image and then save it the next time you open the image in GelCapture the image appears inverted However if you op...

Страница 65: ...or left green arrows or by moving the slider Moving the Min Max sliders will result in different image Min Max gray levels Restore Original Restores any stretching actions to the original image value...

Страница 66: ...he image s grayscale where red refers to the maximum and blue refers to the minimum grayscale levels To View an Image in Pseudo color 1 Click the Pseudo color icon 2 Click the Pseudo color icon again...

Страница 67: ...In Start mode or with a saved image you can crop save copy and view the Histogram The Revert option is only available with a saved image To Define a Region of Interest 1 In Preview Start mode or in a...

Страница 68: ...a Calibration section on page 35 2 Cleaning the Drawer Tray and Unit a The Drawer Tray must be cleaned every time a sample is removed Use a soft non abrasive lint free cloth First dampen with water an...

Страница 69: ...camera drivers Loose power supply cable Verify the wall socket and camera power supply connections Loose USB cable connections Verify the PC and MicroChemi USB connections Camera hardware problems Ple...

Страница 70: ...distributor Button is receiving incorrect voltage Please contact your distributor Improper illumination intensity Possible Reason Action Dimmer dial is set too low so there is not enough light Use th...

Страница 71: ...at one wavelength and triggers the emission of a photon with longer less energetic wavelength making it possible to detect image and measure the amount of fluorescence in samples as small as individua...

Страница 72: ...Features 9 Icons 10 Overview 8 I Imaging Tools 60 Image Statistics 60 Inverted Image 61 Overexposure 62 Pseudo Color 62 Region of Interest 63 Installing Camera Drivers Windows 7 Professional 24 Camer...

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