DNR MicroChemi, Руководство пользователя

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4: Operating Instructions
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b Quantity : choose this option when detailed sample information is needed (e.g., for analysis).
Selecting Quantity will provide the exposure time for producing an image that presents all
the chemiluminescent information.
5 Make your selection according to the requirements and click the Start Capture button to continue
capturing an image, or click Cancel to go back to GelCapture’s main screen.
Binning – Resolution vs. Sensitivity
Live science experiments include samples of both low and high intensity. The Binning function in the
MicroChemi system lets researchers choose various ratios of sensitivity and resolution for a given
image, which will improve the results.
The Binning process takes adjacent pixels and combines them into one giant pixel in order to increase
the camera’s sensitivity. By creating a larger pixel you can “create” a higher ratio of Signal/Noise, for
example, when using Binning to create an area of 4 pixels (2X2 array). This big new pixel, which is
created from 4 normal sized pixels, increases the camera’s sensitivity by four folds, even though the
background noise level remains similar to the level in a normal sized pixel.
A drawback to Binning is the loss of resolution. In order to detect a given image with higher
resolution, you need to decrease the Binning parameters, since as many pixels as possible must be
detected separately.
The following example shows that in the first setting, every pixel in the sensor is detected separately,
thereby providing a high resolution image (1=1). In the second setting every 4 adjacent pixels merge
to form an image that is fourfold more sensitive than the first image, while the resolution decreases
by fourfold. In the third setting, every 9 adjacent pixels merge to form an image that is nine-fold more
sensitive than the first image, while the resolution decreases by nine-fold.