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Operation Manual

DTlite

Real-Time PCR instrument

PART I

Operating Procedure

TS 9443-003-96301278-2010

“DNA-Technology, Research & Production” LLC

Protvino

Содержание DTlite

Страница 1: ...Operation Manual DTlite Real Time PCR instrument PART I Operating Procedure TS 9443 003 96301278 2010 DNA Technology Research Production LLC Protvino...

Страница 2: ...2...

Страница 3: ...hnology Research Production LLC bears no responsibility for any injuries or damage to health caused by unintended use or unauthorized modifications of the instrument Electrical Safety Prior to connect...

Страница 4: ...taff While operation with the instrument wear suitable protective clothing gloves and secure eyes and face do not pipette solutions by mouth do not eat drink smoke or use cosmetic products in the oper...

Страница 5: ...ime_PCR Launch 25 4 2 Preparing the Instrument for PCR 27 4 2 1 Checkup of geometrical settings 27 4 2 2 Optical measurement exposure setting 27 4 2 3 Checking the Cleanness of Thermal Plate Wells 28...

Страница 6: ...me PCR The qualitative analysis is based on evaluation of kinetics of PCR visible part carried out using relevant mathematical tool The use of fluorescent detection of PCR products has a list of subst...

Страница 7: ...nomously On completing the amplification program the instrument goes to the storage mode and if the option Turn off device after the run is activated on the window Run ning the instrument goes to the...

Страница 8: ...1 7 Packing unit RT48 03PG DNA Technology R P 1 The instrument may be delivered along with portable PC with the pre installed software in accordance with customer s request Note PC should be purchased...

Страница 9: ...in tempera ture range of 0 100 modification L 3 0 C s 4 0 C s Average cooling rate within temperature range of 100 55 modification L Maximum cooling rate within temperature range of 100 55 modificatio...

Страница 10: ...nstalled the instrument driver Realtime PCR software to control the instrument and perform data analysis The software may be downloaded for free from the company s official website at http www dna tec...

Страница 11: ...hot lid module The thermal block ensures thermal cycling of the tubes according to the amplification pro gram Two Peltier elements are used for thermal plate cooling and heating providing high ac cura...

Страница 12: ...module is a system of lenses mirrors and light filters that provides folding of the luminous flux from several light sources on one optical axis and its separation from the lumi nous flux entering th...

Страница 13: ...r computer control one can open or close the thermal block using and buttons located under the button in the Running window of RealTime_PCR soft ware LCD screen displays information about an amplifica...

Страница 14: ...bidden are placed on the shipping box as well as trade mark or information about manufacturer brand name date of production net weight gross weight requirements for transportation and storage Symbols...

Страница 15: ...acility in unpacked condition by two persons or using movable table trolley to other preliminary pre pared working place in accordance with corresponding precautions Note In case the instrument is tra...

Страница 16: ...Software installation To control the instrument software from the instrument s delivery set should be installed on PC The software operates in Windows 98 Windows ME Windows 2k XP Windows Vista Windows...

Страница 17: ...puter open Device Manager window then right click DT 964 and select Update driver in the list In the next window Driver Updating select Manual driver search and installation click Browse select Driver...

Страница 18: ...nstallation Select Install from a list of specific location and click Fig 5 Fig 6 Searching Drivers_DT96x folder on the USB flash drive Select a path to the driver folder by ticking Add this location...

Страница 19: ...Drivers_DT96x folder on the USB flash drive and click Fig 7 Making sure the driver folder path is correct Fig 6 click Fig 8 Selecting inf file on the USB flash drive Select the latest version of the...

Страница 20: ...king Fig 9 Select the path to Dt964 sys file on the USB flash drive by clicking Fig 10 Fig 10 Selecting the path to Dt964R sys file on the USB flash drive In the next window Locate file find this file...

Страница 21: ...king sure that the path to the file in the window Fig 10 is correct click After message that the installation of the driver has been completed Fig 12 click Fig 12 Driver installation completion If dri...

Страница 22: ...ch Setup manager from the USB flash drive included in the delivery set it can be also downloaded official website http www dna technology ru eng support Select Russian English language to use during t...

Страница 23: ...ation folder click Select the name of the folder where RealTime_PCR shortcuts will be created and click Fig 16 Fig 16 Selecting of folder to create shortcuts Confirmation window will appear informing...

Страница 24: ...e multi instrument operational conditions the corresponding Serv er_RTDevice service is included in the RealTime_PCR software This service is started at the first start of the software in the mode wit...

Страница 25: ...des Fig 19 Guest operator is a de fault not recommended to use Select another operator by using drop down menu or add a new one drop down menu last line Please type in the name of Operator and select...

Страница 26: ...ince switch on the background color will change from yellow to green the instrument is either switched off at the moment of launching soft ware or is not connected to the PC via USB2 0 A B cable or is...

Страница 27: ...es has to completely encompass the light spots If the circles are displaced relative to the light spots correction of the geometry of an optical image must be carried out Fig 21 Fig 21 Check of geomet...

Страница 28: ...before placing tubes strips or plates with PCR mixes if there was a risk of contamination before Note Routine check of well cleanness must be carried out by the operator in compliance with the instrum...

Страница 29: ...ssay parameters recommended for standard assays in clinical laboratories by using plate protocol templates saved before Note Do not fill in the plate protocol without using Test procedure as window An...

Страница 30: ...ll be displayed as the de fault If the plate protocol was filled in without using the Test procedure the amplification program of the last run will be displayed If required the following steps may be...

Страница 31: ...dit of existing amplification program Creating and editing the amplification program are described in details in the second part of operation manual Software guidance 4 4 Placement of tubes with sampl...

Страница 32: ...control and wait until the thermal block is closed Caution Be sure to place at least 8 tubes in order to prevent their damage because of hot lid pressure If there are less of 8 tubes please add empty...

Страница 33: ...file name data format and folder to save future results of the upcoming RUN Note If Run is started while the instrument is in the process of warming up yellow indicator in the software status line bl...

Страница 34: ...tion of optical measurements over the plate window by clicking Fig 27 Fig 27 Distribution of optical measurements over the plate window If option Turn off device after the run was not activated the in...

Страница 35: ...ary to keep it at optimum working condition which minimises the risk of having unscheduled downtime and providing for its maximum operational life The instrument belongs to IVD medical equipment Maint...

Страница 36: ...han 70 ethanol Caution Never use any metal objects paper clips wire etc for wells cleaning Attention When cleaning the wells and thermo block matrix do not allow a liquid flow ing inside of the therma...

Страница 37: ...stru ment and PC Instrument driver is not installed Install the instrument driver see item 3 3 1 Failure in the Win dows system Reset the PC After 10 to 15 minute warm up process of the in strument th...

Страница 38: ...on mechanical parts To avoid their damage during han dling and shipping the requirements of manipulation signs applied on package should be strictly followed Attention The instrument has to be shipped...

Страница 39: ...e of charge by repairing or replacing it on a similar one provided that the occurred defects are fault of the manufacturer Execution the warranty repair of the failed instrument by the manufacturer en...

Страница 40: ...40...

Страница 41: ...he packed product is accepted by ____________________ signature L S Note The form is to be filled in at the factory that packed the product 11 Acceptance certificate DTlite Real Time PCR instrument Se...

Страница 42: ...42...

Страница 43: ...______ manufacturer s name 20 manufacturer s stamp date personal signature Owner and his address personal signature CARD No 2 to be filled in by the manufacturer For warranty repair maintenance of DTl...

Страница 44: ...ct factory No Repair content Date of repair day month year Serviceman Owner signature stamp signature Reverse of CARD No 2 to be filled in by a service center Product factory No Repair content Date of...

Страница 45: ...____________________ Means used for disinfection _________________________ ___________________________________________________________________________ _________________________________________________...

Страница 46: ..._______________________________________________ ________ _______________________________________________________________________________ ________ ______________________________________________________...

Страница 47: ...47...

Страница 48: ...E mail info dna technology com Customer support Tel 8 800 200 75 15 free for Russia E mail hotline dna technology ru Feedback form see on DNA Technology s website http www dna technology ru customer_s...

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