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Fill the p1 schott bottle with a maximum of 300 ml of buffer. In the pressure interface menu set the
pressure p1 and p2 as follow:
P1 = 150mm Hg
P2 = 70mm Hg
Set the Pressure ON
Set Flow ON
IMPORTANT:
• For 114P Users continue as below and for 112PP, users go to section 9
• Let the pressure regulator run until no air bubbles are visible in the perfusion circuit.
• NEVER RUN AIR BUBBLES THROUGH A VESSEL AS THIS MAY DAMAGE IT
NOTE: Never fill the buffer bottle with more than 300 ml as higher volumes makes precise
control of the pressure more difficult.
1. When no air is seen in the tubing turn of the flow and close the P1 3-way valve to the chamber as
shown below.
Connected to P1 Reservoir Bottle
2. Fill the 114P chamber with 6ml pre-heated buffer.
3. Detach the short silicone tubing from the short steel pipe connecting the P1 glass cannula to the steel
pipe inside the chamber.
FOR 114P USERS CONTINUE AS BELOW AND FOR 112PP, USERS GO TO SECTION 9
