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2.1 SETTING UP THE COMPLETE PRESSURE MYOGRAPH SYSTEM

Figure 2.1 is an example of a complete set-up for the Pressure Myograph System. The overview includes
optional equipment such as a peristaltic pump to enable superfusion flow and pH probes for measuring
pH in the superfusion buffer. In addition, the overview also includes the DMT Flowmeter - 162FM. The
Flowmeter enables flow measurements in the range of 15-4000μl/min. A detailed step-by-step description
is provided in the remaining sections of chapter 2.

The Pressure Myograph System will work with a range of different microscopes: the DMT Microscope,

Figure 2.1 Example of a complete set-up of Pressure Myograph System with DMT inverted Microscope

Gas supply

Oxygen/

Carbogen/

Compressed

air

Waste Bottle

pH electrode

FlowMeter

162FM

P1

P2

PRESSURE INTERFACE

(REAR PANEL)

Electronic

vacuum valve

Pressure Myograph

Oxygen/

Carbogen/

Compressed air

Power

supply

4 Recorder

outputs

(Analogue)

USB output

(Digital)

Myoview

Waste Bottle

Peristaltic

pump

Oxygen/Carbogen/
Compressed air

Vacuum

Pump

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Содержание MYOGRAPH SYSTEM 112PP

Страница 1: ...USER MANUAL VOL 2 0 M Y O G R A P H S Y S T E M 1 1 2 P P 1 1 4 P ...

Страница 2: ......

Страница 3: ...scribes since then New information may be supplied separately This documentation is provided with the DMT Automated Multi Wire Myograph System Model 630MA All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A S Every attempt is made to ensure accurate information misprints construction and s...

Страница 4: ... Pressure Myograph System 13 2 2 Setting up step by step 15 2 3 The first force and pressure calibrations 17 2 4 External Pressure Myograph connections 18 CHAPTER 3 PRESSURE INTERFACE MENUS 30 3 1 General description of how to navigate the touch screen 30 3 2 Power up screen 31 3 3 MAIN MENU 32 3 4 Pressure Menu 34 3 5 Heat Menu 36 3 6 Timer and Buzzer Menu 37 3 7 Setting Menu 38 ...

Страница 5: ...fects and mistakes due to wrong connections void warranty We are not accountable for results and mistakes due to inappropriate pressure hookup Do not install near any heat sources such as radiators heat registers stoves or other apparatus that produce heat Only use attachments and accessories specified by the manufacturer Unplug this apparatus during lightning storms or when unused for long period...

Страница 6: ...nts of the EMC Directive 2004 108 EC Based on the following specifications applied by EN 61326 1 2006 EN 61326 2 6 2006 EN 61326 2 6 Corr 2007 And with the LVD Directive 2006 95 EC Based on the following specifications applied by EN 61010 1 2010 EN 61010 2 030 2010 General warnings regarding EMC Do not use this device in close proximity to sources of strong electromagnetic radiation e g unshielded...

Страница 7: ... list to check that the system is complete Pressure Interface Pressure Interface to chamber cable with a temperature probe Power adapter the shape of the AC plug varies by country be sure that the plug has the right shape for your location USB cables for PC connection Atlas Copco Cablair hose for main pressure supply Two 500ml Schott Duran Bottles with specially designed screw caps Pressure Myogra...

Страница 8: ...um grease Tube of grease for linear slides Silicone tubing internal diameter 1mm Computer hardware software manuals USB digital camera optional Computer optional CD with MyoVIEW software program optional CD with User Manual for Pressure Myograph Systems and User Guide for 114P or 112PP ...

Страница 9: ...9 CHAPTER 1 SYSTEM OVERVIEW 1 1 INTERFACE FRONT PANEL Figure 1 1 Pressure Interface front 2 x 500 ml Schott Duran bottles Power indicator Touch display ...

Страница 10: ...Schott Duran bottles ON OFF switch 24V DC port for power Analogue recorder output connection to external data acquisition software optional USB connection port for computer Connection port for pH electrode optional Ground connection Inlet for oxygen carbogen to pressure bottles max 1 bar ...

Страница 11: ... Outlet valve Left Glass Cannula Chamber Window Right Glass Cannula Silicone tube connecting perfusion inlet pipe and right Glass Cannula Perfusion inlet pipe Fixation plate for right Glass Cannula Fixation plate for left Glass Cannula Screw for adjusting the P2 arm in the vertical direction Outlet connecting pipe Silicone tube connecting perfusion outlet pipe and right Glass Cannula Outlet pressu...

Страница 12: ...Chamber Window Right Glass Cannula Silicone tube connecting perfusion inlet pipe and right Glass Cannula Perfusion inlet pipe Fixation plate for right Glass Cannula Fixation plate for left Glass Cannula Screw for adjusting the P2 arm in the vertical direction Outlet connecting pipe Silicone tube connecting perfusion outlet pipe and right Glass Cannula Pressure Release screw for Pulsation Chamber1 ...

Страница 13: ...min A detailed step by step description is provided in the remaining sections of chapter 2 The Pressure Myograph System will work with a range of different microscopes the DMT Microscope Figure 2 1 Example of a complete set up of Pressure Myograph System with DMT inverted Microscope Gas supply Oxygen Carbogen Compressed air Waste Bottle pH electrode FlowMeter 162FM P1 P2 PRESSURE INTERFACE REAR PA...

Страница 14: ...n setups requiring standard image analysis The Zeiss Axio vert A1 is an inverted microscope that provides the highest degree of flexibility in image analysis The Zeiss Axio vert A1 a Nikon TS 100F and Motic AE 2000 enable the use of a wide range of objectives and facilitate sophisticated fluorescence techniques Contact the DMT Sales Department for further product information and price on any optio...

Страница 15: ...re Interface to the USB port on the computer 3 Pressure Interface and oxygen supply connection Connect the Oxygen inlet on the back of the Pressure Interface to an adjustable oxygen supply using the Atlas Copco Cablair hose delivered with the Pressure Myograph System 4 Microscope camera to computer connection A DMT Inverted Microscope Inside the DMT Microscope there is a USB camera wired to a USB ...

Страница 16: ...xternal Data Acquisition System connection optional If you have not obtained the MyoVIEW software the four BNC ports on the rear of the Pressure Interface enables connection of an external data acquisition system for recording inlet pressure P1 outlet pressure P2 longitudinal force probe temperature chamber temperature flow ON OFF pressure ON OFF pH1 value and Optional Dig out1 Dig out2 Dig in1 di...

Страница 17: ...uous testing including final force and pressure calibrations However in order to ensure that the Pressure Myograph System is working at highest performance DMT recommends that new force and pressure calibrations are performed before the first use The force and pressure calibration procedures are described in detail in chapter 3 7 1 ...

Страница 18: ...n the Pressure Myograph see figure 2 2 and 2 3 for Pressure Myograph 114P and Pulsatile Pressure Myograph 112PP respectively to the P1 outlet on the cap of the Pressure Interface bottle see figure 2 4 Connect the perfusion outlet at P2 on the Pressure Myograph see figure 2 2 and 2 3 for Pressure Myograph 114P and Pulsatile Pressure Myograph 112PP respectively to the P2 inlet on the Pressure Interf...

Страница 19: ...essure Myograph Example with Pressure Myograph 114P Perfusion outlet P2 Suction pipe for vacuum Superfusion outlet Inlet pipe for Oxygen carbogen supply Perfusion inlet P1 Extra Perfusion inlet Access hole for temperature or pH probe Superfusion inlet pipe Access hole for adding buffer and drugs Extra Perfusion outlet ...

Страница 20: ...alibration shunt resistance tube as shown below and described in chapter 3 7 2 1 Inlet pipe for Oxygen carbogen supply Figure 2 5 Mounting of the Calibration Shunt tube in the 114P and 112PP chamber Calibration Shunt tube delivered in a small box with the system ...

Страница 21: ... BUBBLES THROUGH A VESSEL AS THIS MAY DAMAGE IT NOTE Never fill the buffer bottle with more than 300 ml as higher volumes makes precise control of the pressure more difficult 1 When no air is seen in the tubing turn of the flow and close the P1 3 way valve to the chamber as shown below Connected to P1 Reservoir Bottle 2 Fill the 114P chamber with 6ml pre heated buffer 3 Detach the short silicone t...

Страница 22: ...the steel pipe inside the chamber again 5 Close the P2 3 valve towards the chamber as shown below Connected to port of Pressure Interface marked P2 6 Detach the silicone tube on the top of the chamber at the P2 side as the position shown in the figure below with a blue circle Attach a 5ml syringe with an 18 gauge needle to the silicone tube and use the syringe to drag buffer into the P2 glass cann...

Страница 23: ...erfusion Inlet and push a small volume of buffer through the 3 way valve to remove air in the valve Then close the 3 way valve toward the P1 buffer flask as shown below 10 Now very gently with the syringe push buffer into the chamber MONITOR the P1 Pressure on the Pressure Interface screen and DO NOT exceed 200mmHg Push 1ml into the chamber and P1 cannula to remove air Close the 3 way valve toward...

Страница 24: ...ntil no air bubbles are visible in the perfusion circuit NOTE Never fill the buffer bottle with more than 300 ml as higher volumes makes precise control of the pressure more difficult IMPORTANT NEVER RUN AIR BUBBLES THROUGH A VESSEL AS THIS MAY DAMAGE THE ENDOTHELIUM 14 When no air is seen in the tubing turn of the flow and close the P1 3 way valve to the chamber as shown below Connected to P1 Res...

Страница 25: ... the steel pipe inside the chamber again 18 Close the P2 3 valve towards the chamber as shown below Connected to port of Pressure Interface marked P2 19 Detach the silicone tube on the top of the chamber at the P2 side at the position shown in the figure below with a red circle Attach a 5ml syringe with an 18 gauge needle to the silicone tube and use the syringe to drag buffer into the P2 glass ca...

Страница 26: ...les at the 3 way valves 22 Attach a 5 10ml syringe at the extra P1 perfusion Inlet and push a small volume of buffer through the 3 way valve to remove air in the valve Then close the 3 way valve toward the P1 buffer flask as shown below 23 Now very gently with the syringe push buffer into the chamber MONITOR the P1 P2 and P4 Pressure on the Pressure Interface screen and DO NOT exceed 200mmHg in th...

Страница 27: ...the introduction of special agents or reagents to the perfusion circuit during an experiment They are placed at P1 and P2 on the Pressure Myograph and the small handle on top of the valves controls the flow direction See figure 2 6 Figure 2 6 3 way valve flow settings at P1 2 4 3 CONNECTING SUPERFUSION FLOW OPTIONAL Creating a superfusion flow in the Pressure Myograph chamber requires an external ...

Страница 28: ...a will make it possible to record live traces of the flow dependent parameters as shear stress and vascular resistance due to changes in vessel structure and function in various physiological and pharmacological studies To add the stand alone FlowMeter to the Pressure Myograph System the cable from the flow is attached to the Flowmeter port marked on the backside of the Pressure Interface MyoVIEW ...

Страница 29: ...rated in figure 2 1 The internal diameter of the silicone tube used for this connection must be 2 mm a 2 mm tube is not included with the Pressure Myograph System Figure 2 7 Description of coverlid functions for the 114P and 112PP chamber Vacuum suction Superfusion inlet pipe Oxygen carbogen supply pipe Temperature or pH probe hole Superfusion outlet pipe Buffer drug supply hole ...

Страница 30: ...creen To access a menu simply touch the screen When a setting needs to be changed press SELECT beside the line to be changed The line selected will turn blue indicating that the Pressure Interface is in edit mode and waiting for input When DEFAULT is chosen a default value will be displayed DEFAULT To change the numeric value for the selected parameter touch the UP or DOWN arrows Pressing the whit...

Страница 31: ...fter turning on the Pressure Interface an introduction screen appears It displays the software version number While this screen is displayed the system is auto initializing After a few seconds the Main Menu display will appear ...

Страница 32: ... including the time heat flow and pressure See next page for a description of the Main Menu Four sub menus are accessible from the Main Menu screen Pressure Menu Heat Menu Timer and Buzzer Menu Setting Menu Icon for Pressure ON OFF Icon for FLOW ON OFF Icon for Heater ON OFF Timer Icon for Buzzer ON OFF If ON the icon is green Pressure Menu Heat Menu Timer Buzzer Menu Settings Menu ZERO Is used to...

Страница 33: ...er heat If the icon is grey the heat is OFF If the icon is green the heat is ON active OFF ON Flow valve icon This icon indicate the status on the flow valve If the icon is grey the flow valve is OFF NO flow If the icon is green the flow valve is ON active OFF ON Pressure icon This icon indicates the status of the pressure regulation If the icon is grey the pressure regulation is OFF If the icon i...

Страница 34: ... is setting on the inlet side of the Pressure Myograph Pressure range is 0 250mmHg P2 Outlet setpoint is the pressure value that the regulator is setting on the outlet side of the Pressure Myograph Pressure range is 0 250mmHg Pressure gradient set the speed at which the regulator modifies the pressure The pressure change range is 1 10mmHg sec IMPORTANT P1 inlet setpoint should be higher than p2 ou...

Страница 35: ...r on press ON outside the line for Pressure When activated it turns green In Main Menu the pressure icon also turns green when pressure regulation is on ON To turn the flow on press ON outside the line for Flow When activated it changes green In Main Menu the flow icon also turns green when flow is on ON ...

Страница 36: ...ser to change the system temperature as well as turn heat on or off Pressing DEFAULT will automatically reset the temperature setpoint to 37 C Manually change the temperature by pressing the UP or DOWN arrows Pressing ENTER will save the new temperature setpoint To turn the heat on press ON and it will turn green The system will heat to the desired temperature setpoint In Main Menu the thermometer...

Страница 37: ...gram the timer The hours are programmed first and then use the RIGHT arrow to get to the minutes and then the seconds Pressing ENTER will save the programmed time To start the timer press the Timer ON and it will turn green When the timer reaches zero it automatically switches to OFF If the buzzer is set ON it will make a tone when the timer reaches zero To activate the buzzer press ON and it will...

Страница 38: ...ut Interface Settings Pulse Rate Cleaning Menu Select 114P 112PP 3 7 1 FORCE CALIBRATION MENU Prior to shipping the Pressure Myograph System has gone through two days of continuous testing including final force calibrations However DMT recommends that new force calibrations are performed before starting to use the Pressure Myograph System for the first time The calibration procedure needs to be pe...

Страница 39: ...ixation plate as illustrated in figure 3 1 and 3 2 for Pressure Myograph 114P or Pulsatile Pressure Myograph 112PP respectively Carefully move the calibration bridge until the tip of the T balance pin is placed freely and properly in place Press NEXT STEP Enter Force Calibration Menu by pressing Settings in the Main Menu and then press Force Calibration Menu to begin the transducer calibration The...

Страница 40: ...40 Figure 3 1 Illustration of how to fit the T balance just behind the P2 Pipette holder arm ...

Страница 41: ...hamber are heated and have reached the target temperature Step 4 This is the first step in the actual force calibration process A four digit number will be displayed in blue at the bottom of the screen If nothing has been disturbed during the heating process the zero 0 gram calibration should be stable After 30 to 45 seconds the four digit number will not fluctuate If the four digit number is not ...

Страница 42: ...ressure Myograph System is now ready for longitudinal force measurements 3 7 2 PRESSURE CALIBRATION MENU 3 7 2 1 Set up the pressure calibration procedure Press Settings in the Main Menu and then press Pressure Calibration The set up to perform the pressure transducer calibration procedure is illustrated in figure 3 2 Carefully follow the procedure described below to calibrate the Pressure Myograp...

Страница 43: ...teel pipe 4 Carefully disconnect the silicone tube A between the outlet connecting pipe and the outlet pressure transducer connecting pipe see figure 3 3 5 Mount the enclosed calibration shunt resistance tube to the outlet connecting pipe A and the stainless steel pipe B as illustrated in figure 3 4 Rem ove this silicone tube carefully not breaking the force transducer B Remove this silicone tube ...

Страница 44: ...P1 3 7 2 2 Set up the pressure calibration on the Pressure Interface for the 114P System Press Settings in the Main Menu and then press Pressure Calibration The set up to perform the pressure transducer calibration procedure is illustrated in figure 3 2 Carefully follow the procedure described below to calibrate the Pressure Myograph System The calibration procedure is listed in 5 individual steps...

Страница 45: ...lues stabilize go to NEXT STEP The relative values should be between 3900 to 4500 Step 4 Apply a pressure of 200mmHg to the Pressure Myograph using the Big Ben Pressure Manometer When the 3 blue lines of relative values stabilize go to NEXT STEP The relative values should be between 4600 to 5200 Step 5 The calibration is now finished The readings in the three bottom lines are the actual pressure o...

Страница 46: ... NEXT STEP The relative values should be between 3300 to 3900 Step 3 Apply a pressure of 125mmHg to the Pressure Myograph using the Big Ben Pressure Manometer When the 4 blue lines of relative values stabilize go to NEXT STEP The relative values should be between 3900 to 4500 Step 4 Apply a pressure of 200mmHg to the Pressure Myograph using the Big Ben Pressure Manometer When the 4 blue lines of r...

Страница 47: ... up Menu The temperature is an important parameter in the calibration formula and is obtained automatically if AUTO is selected in Temperature compensation function If Manual is selected the manual temperature is used in the pH calibration formula and the temperature probe is de activated In the Manual mode the temperature of the calibration buffers is measured with a thermometer and entered manua...

Страница 48: ...ut in the blue line is stable go to NEXT STEP Step 3 Place the pH electrode and temperature probe in the low buffer solution here pH 4 and turn on stirring of the low buffer solution When the relative pH output in the blue line is stable go to NEXT STEP Step 4 The calibration is now finished The values in the two bottom lines are the actual pH and temperature reading ...

Страница 49: ...et up Use SELECT to select the line to be changed Use Up and DOWN arrows or DEFAULT to change select a new set up The following parameter in the Pressure Interface can be selected as output on the analog channel Force probe temperature chamber temperature pH1 pressure regulation ON OFF Flow ON OFF Optional Digital output 1 Digital output 2 Digital input 1 Digital input 2 Pressure P1 Pressure P2 Wh...

Страница 50: ...mber and bottle individually so the exact temperatures can be reached This is referred to as a temperature offset TEMP OFFSET on chamber Pressing SELECT or ALL have the same function in this menu as previously described Pressing ENTER will save the values for future experiments 3 7 5 2 pH Set up Menu The temperature is an important parameter in the calibration formula and is obtained automatically...

Страница 51: ...ering the proper five digit login code will give the trained technician access to diagnostics panels that will provide information during a malfunction or mechanisms to change other settings controlled by the on board computer 3 7 6 SELECT 114P 112PP The Select 114P 112PP Menu is only for users that have an 114P and a Pulsatile Pressure 112PP chamber Using this menu the user can change the 114P Pr...

Страница 52: ...the P2 pressure Changing the Duty Pulse High value will change the pulsing profile if wanted PULSATION Pulsation is turned ON or OFF All the above settings can be controlled in the MyoVIEW 4 0 software 3 7 8 CLEANING MENU ONLY PULSATILE 112PP The Cleaning Menu is mandatory to use when the system is filled purged with running buffer or cleaned In the Cleaning Menu the user is able to monitor all fo...

Страница 53: ...3 When the Pressure exceed 200mmHg the number will turn red to indicate that the pressure applied manually is close to the maximum pressure the system can handle without breaking the pressure transducers ...

Страница 54: ...RE Range 15 0 to 50 0 C Resolution 0 1 C Stability 0 2 C Heating Yes TRANSDUCER FORCE Output reading mN Range 200 mN Resolution 0 01 mN Force calibration Yes TRANSDUCER PRESSURE Output reading mmHg Range 0 250 mmHg Pressure stability 0 5 mmHg Resolution 0 1 mmHg Force calibration Yes RESERVOIR Heated Yes Capacity 250 ml Pressure circuit Closed Air inlet 1 bar max OUTPUT Data communication USB 2 0 ...

Страница 55: ...Stability 0 2 C Heating Yes TRANSDUCER FORCE Output reading mN Range 200 mN Resolution 0 01 mN Force calibration Yes TRANSDUCER PRESSURE Output reading mmHg Range 0 250 mmHg Pressure stability 0 5 mmHg Resolution 0 1 mmHg Force calibration Yes RESERVOIR Heated Yes Capacity 250 ml Pressure circuit Closed Air inlet 1 bar max OUTPUT Data communication USB 2 0 Analogue output channels 4 Analogue outpu...

Страница 56: ...486 9685 Fax 86 21 5877 0063 DMT Asia Pacific Tel 61 2 8814 1597 Danish Myo Technology A S E mail sales dmt dk Tel 45 87 41 11 00 Fax 45 87 41 11 01 DMT USA Inc E mail sales dmt usa com Tel 1 734 707 0250 Fax 1 678 302 7013 ...

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