27
24. The 114P chamber can now be moved to a dissection microscope for mounting of the vessel in the
chamber by detaching the silicone tubes connected to the P1 and P2 3-way valves.
25. The 114P/112PP chamber can now be moved to a dissection microscope for mounting an artery in
the chamber.
NOTE: Artery mounting procedure see “pressure myograph -114p/112pp – user guide
page 6-8”.
2.4.2 EXTRA PERFUSION INLET/OUTLET
The extra perfusion inlet/outlet 3-way valves on the Pressure Myograph allow the introduction of special
agents or reagents to the perfusion circuit during an experiment. They are placed at P1 and P2 on the
Pressure Myograph and the small handle on top of the valves controls the flow direction. See figure 2.6.
Figure 2.6 3-way valve flow settings at P1
2.4.3 CONNECTING SUPERFUSION FLOW (OPTIONAL)
Creating a superfusion flow in the Pressure Myograph chamber requires an external peristaltic pump,
which is not part of the basic Pressure Myograph System. Contact DMT for further information about
recommended perfusion pump models, specifications and prices. The superfusion circuit is connected
to the Pressure Myograph cover lid steel pipes as illustrated in figure 2.7.
