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CHAPTER 3 - EXPERIMENTAL SET-UP
This chapter contains experimental set-up for the
Wire Myograph. For dissection of a vessel, please
see Procedures for investigations of small vessels
using a small vessel Myograph by M.J. Mulvany.
3.1 MOUNTING PROTOCOL FOR SMALL ARTERIES
The procedure involves attaching the mounting
wires to jaws which are in turn mounted on the
force transducer. This force transducer is capable
of measuring with a sensitivity of about 0.01 mN
(1 mg), but can be damaged if the applied force
exceeds about 1600 mN (160 g). Therefore, care
must be taken to avoid pressing the jaws too hard
together. A movement of ~20 µm after they have
touched is sufficient to hold the wires clamped.
3.1.1 MOUNTING STEP ONE
• Cut lengths of 40 μm wire ~2.2 cm long.
Mount one wire on left-hand jaw of the Wire
Myograph as follows.
• Holding wire at far end, place center of wire
between jaws and screw jaws together so
that the wire is clamped (figure 3.1 A).
• Bend the far end of the wire towards the left,
and wrap it around under fixing screw, so the
wire is wound clockwise: tightening the screw
will then tighten the wire. This procedure
should result in the wire being clamped
between the jaws and with near end of wire
pointing towards operator (figure 3.1 B-C).
• Fill the Wire Myograph chamber with PSS
(at room temperature). See appendix 1 for
example of buffer recipes.
WARNING: Do NOT close the jaws
too hard against each other.
Figure 3.1 A, B and C Mounting step 1
A
B
C
Содержание 630MA
Страница 1: ...USER GUIDE MYOGRAPH SYSTEM 630MA ...
Страница 2: ...AUTOMATED MULTI WIRE MYOGRAPH SYSTEM ...
