ICS-90 Installation Instructions
32
Doc. 031856-03 12/06
e.
Using a stopwatch or clock’s second hand, time the flow of eluent into the
syringe for 60 seconds. Determine the volume collected by noting the
beginning and ending positions of the syringe plunger.
f.
The volume of eluent collected in 60 seconds should be approximately
equal to the desired flow rate in milliliters per minute. For example, for a
flow rate of 1 mL/min, the volume should be 1.0
±
0.1 mL.
g. If necessary, adjust the flow rate knob, and then repeat the measurement.
Continue adjusting until the desired milliliters per minute is collected.
h. Reconnect the
ELUENT IN
line to the suppressor.
Volumetric Method 2: Using a Graduated Cylinder
a.
Disconnect the
ELUENT IN
line from the
ELUENT IN
port on the
suppressor.
b. Using a clock or watch with a second hand (the computer’s clock can be
used), collect eluent from the
ELUENT IN
line into a small graduated
cylinder for 2 minutes.
c.
The collected eluent should be approximately equal to the desired flow
rate in milliliters per minute. For example, for a flow rate of 1 mL/min,
2.0
±
0.2 mL should be collected in 2 minutes.
d. If necessary, adjust the flow rate knob, and then repeat the measurement.
Continue adjustments until the desired eluent per minute is collected.
e.
Reconnect the
ELUENT IN
line to the suppressor.
Gravimetric Method
a.
Disconnect the
ELUENT IN
line from the
ELUENT IN
port on the
suppressor.
b. Using a tared beaker and a calibrated stopwatch, collect the eluent from
the
ELUENT IN
line for 60 seconds.
c.
Weigh the mass of the eluent. The mass (in grams) should be
approximately equal to the desired flow rate in milliliters per minute. For
example, for a flow rate of 0.5 mL/min, the mass of the collected eluent
should be 0.5
±
0.02 g.
d. If necessary, adjust the flow rate knob, and then repeat Steps b and c.
Continue adjusting until the desired mass per minute is collected.
e.
Reconnect the
ELUENT IN
line to the suppressor.
