Biotek Synergy LX, Руководство пользователя

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Fluorescence Specifications | 112
Fluorescence Specifications
Detection limit: Sodium Fluorescein in
phosphate buffered saline (PBS) DL ≤20 pM, Excitation 485/20 nm, Emission
528/20 nm, 510 nm mirror
Detection limit: Methylumbelliferone (MUB)
in carbonate-bicarbonate buffer (CBB) DL ≤0.16 ng/mL (0.91 nM), Excitation 360/40
nm, Emission 460/40 nm, 400 nm mirror
Minimum kinetic interval:
< 55 seconds for a 96-well microplate with the
following read settings:
Delay after plate movement: 100 ms
Measurements per data point: 10
Wavelength range:
320–700 nm (low-noise PMT)
320–850 nm (red-shifted PMT)
Luminescence Specifications
Production testing is performed using a Harta plate.
96-well plate with standard low-noise PMT
Detection Limit for ATP: ≤ 75 amol/well
Integration Time: 10 seconds
PMT Gain: 150
Blank Wells: 16
Wavelength Range: 200–700 nm
96-well plate with red-shifted PM
Detection Limit for ATP: ≤ 500 amol/well
Integration Time: 10 seconds
PMT Gain: 150
Blank Wells: 16
Wavelength Range: 200–850 nm
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.