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111 | Appendix A : Specifications
Linearity
By liquid dilution.
96-well plate, normal read speed: 0.000 to 2.000 OD: ±1.0% ±0.010 OD, delay after plate
movement = 100 ms
2.000 to 2.500 OD: ±3.0% ±0.010 OD, delay after plate
movement = 100 ms
384-well plate, normal read speed: 0.000 to 1.500 OD: ±2.0% ±0.010 OD, delay after plate
movement = 100 ms
1.500 to 2.000 OD: ±5.0% ±0.010 OD, delay after plate
movement = 100 ms
96- and 384-well plate, sweep read
speed: 0.000 to 1.000 OD: ±1.0% ±0.010 OD
Repeatability (Standard Deviation [STD])
Tested with certified neutral density glass measured by one standard deviation (8
measurements/data point)
96-well plate, normal read speed: 0.000 to 2.000 OD: ±1.0% ±0.005 OD, delay after plate
movement = 100 ms
2.000 to 2.500 OD: ±3.0% ±0.005 OD, delay after plate
movement = 100 ms
384-well plate, normal read speed: 0.000 to 1.500 OD: ±1.0% ±0.005 OD, delay after plate
movement = 100 ms
1.500 to 2.000 OD: ±3.0% ±0.005 OD, delay after plate
movement = 100 ms
96- and 384-well plate, sweep read
speed: 0.000 to 1.000 OD: ±2.0% ±0.010 OD
Assay Validation Using a Take3 Plate
260 nm dsDNA detection limit: < 5 ng/μL
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.