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Sequence BioTech,

LLC

Table of Contents

tWo-YeAr WArrANtY ................................................................ 1

SAFeGUArDS ..................................................................................... 3

GENERAL INFORMATION

..................................................................... 4

UNPACKiNG tHe PiPettor ......................................................... 4

ProDUCt oVerVieW .................................................................... 5

PArtS oF tHe PiPettor ............................................................. 6

reCoMMeNDeD PiPette tiPS ................................................... 7

SET UP AND INSTALLATION

............................................................... 8

iNStALLiNG tHe PiPettor ......................................................... 8

ADJUStiNG PiPettor HeAD ASSeMBLY .............................. 9

ADJUStiNG ASPirAtioN AND DiSPeNSiNG SPeeDS ....... 10

ADJUStiNG tiP rACK HoLDer .................................................. 11

USe oF 384 WeLL MiCrotiter PLAteS ............................... 12

BASIC OPERATION

................................................................................. 13

BASiC oPerAtioN BUttoNS ..................................................... 13

GeNerAL PiPettiNG Set-UP ...................................................... 15

BASiC ProGrAMMiNG iNStrUCtioNS .................................. 17

MAINTENANCE

......................................................................................... 19

reGULAr MAiNteNANCe ............................................................ 19

CLeANiNG AND DeCoNtAMiNAtioN ..................................... 20

HANDLING AND ENVIRONMENTAL CONDITIONS

................... 21

HANDLiNG tHe PiPettor ........................................................... 21

eNViroNMeNtAL ............................................................................. 22

APPENDIX

................................................................................................... 23

SPeCiFiCAtioNS ............................................................................... 23

DeCLArAtioN oF CoNForMitY .............................................. 24

ProGrAMMiNG CoMMANDS AND ACtioNS ....................... 25

ProGrAMMiNG eXAMPLeS ......................................................... 26

SERVICE PLANS

....................................................................................... 29

PLANS A–C .......................................................................................... 29

PLAN D .................................................................................................. 30

PLAN SPeCiFiCS ............................................................................... 31

MANUAL 54652

Содержание Sequence Precision Series

Страница 1: ...Accelerate Liquid Transfers 96 384 BenchTop Pipettor www sequencebiotech com Instruction Manual PRECISION SERIES PIPETTOR PRECISION SERIES Pipettor ...

Страница 2: ...ed menu features The Precision Series Pipettor is an intuitive multichannel pipettor capable of 96 and 384 well formats Plate stamping plate reformatting mixing and serial dilute have never been so easy and quick 2 2 2 Improved accuracy 2 Improved accuracy electronics updates 2 electronics updates ...

Страница 3: ... 10 ADJUStiNG tiP rACK HoLDer 11 USe oF 384 WeLL MiCrotiter PLAteS 12 BASIC OPERATION 13 BASiC oPerAtioN BUttoNS 13 GeNerAL PiPettiNG Set UP 15 BASiC ProGrAMMiNG iNStrUCtioNS 17 MAINTENANCE 19 reGULAr MAiNteNANCe 19 CLeANiNG AND DeCoNtAMiNAtioN 20 HANDLING AND ENVIRONMENTAL CONDITIONS 21 HANDLiNG tHe PiPettor 21 eNViroNMeNtAL 22 APPENDIX 23 SPeCiFiCAtioNS 23 DeCLArAtioN oF CoNForMitY 24 ProGrAMMiN...

Страница 4: ...cessories improper or inadequate maintenance by the user installation of the instrument or use of products not authorized by Sequence BioTech use with incompatible liquids or other materials and modification or repair of the instrument not authorized by Sequence BioTech in addition the warranty is void if the serial number has been altered or defaced Maintenance is the owner s responsibility Promp...

Страница 5: ...tieS oF MerCHANtABiLitY FitNeSS For A PArtiCULAr PUrPoSe or oF NoN iNFriNGeMeNt WHetHer AriSiNG FroM A StAtUte or otHerWiSe iN LAW or FroM A CoUrSe oF DeALiNG or USAGe oF trADe or otHerWiSe ALL oF WHiCH Are eXPreSSLY DiSCLAiMeD tHe reMeDieS ProViDeD HereiN Are tHe BUYer S SoLe AND eXCLUSiVe reMeDieS For BreACH oF WArrANtY No AGeNt eMPLoYee or rePreSeNtAtiVe oF SeLLer HAS ANY AUtHoritY to MoDiFY tH...

Страница 6: ...mage to the instrument Use only for its intended purpose as described in this manual Do not use attachments and accessories not recommended by the manufacturer Do Not operate this instrument if it is damaged in any way Keep this instrument away from heated surfaces Do Not turn the unit upside down the Precision Series Pipettor must always rest on its Base to avoid damaging internal components or b...

Страница 7: ... Not SPeCiFieD BY tHe MANUFACtUrer USe oNLY ACCeSSorieS AND DiSPoSABLeS reCoMMeNDeD BY tHe MANUFACtUrer ACCUrACY AND oPerAtiNG LiFetiMe MAY Be AFFeCteD iF ALterNAtiVe ProDUCtS Are USeD ANY DAMAGe CAUSeD BY NoN reCoMMeNDeD ProDUCtS Are Not CoVereD BY tHe MANUFACtUrer S WArrANtY This instruction manual includes all required information regarding unpacking installing operating programming and maintai...

Страница 8: ...cally centered by manually sliding the pipette Head Assembly horizontally raising and lowering pipette tips into the microplates is performed by simply moving the Levered Handle vertically the head and tip assembly is precisely counter balanced to make vertical motion effortless the entire process including picking up tips aspirating from one location and dispensing to another is accomplished with...

Страница 9: ...g Spring loaded plunger engages for shipping and or transporting 7 Height Adjustment Posts 2 Sets depth of pipettor Head Assembly 8 Side Handles 2 Use to pick up the instrument located on each side of the Base 9 384 Plate Adaptor Use when pipetting into 384 well plates otherwise remove from platform 10 tip rack Holder Secures pipette tip rack 11 Base three position Base 12 Bearing rail For easy ho...

Страница 10: ... inc products For further information email info sequencebiotech com refer to the product specification sheet visit www sequencebiotech com or contact your local representative cat Description Vol Quantity 10330 OneTouch Non Sterile 250 uL 960 pk 5 packs per case 200 uL Pipette tip 10340 OneTouch Sterile 250 uL 960 pk 5 packs per case 200 uL Pipette tip 10350 OneTouch Sterile 250 uL 960 pk 5 packs...

Страница 11: ... and down with minimal effort using the front Levered Handle 2 Unlock Horizontal Motion Pull up on the Locking Key Ring b and rotate it 90 this places the spring loaded plunger in the unlocked position the instrument is now free to move side to side 3 Power Up Plug the 24 VDC power supply into an AC electrical outlet insert the plug on the back of the instrument c and screw the plug collar onto th...

Страница 12: ... under the Chassis Assembly Place a microtiter plate in either the center or right plate position and lower the Head Assembly using the Levered Handle until the desired height is achieved Rotate the Height Adjustment Posts until they touch the Chassis Assembly Horizontal Motion The Chassis Assembly is automatically aligned and perfectly centered over each well position Grab the Levered Handle and ...

Страница 13: ...MENU on the Control Panel rotate the center black Control Wheel until Options appears on the display screen then select ENTER rotate the Control Wheel to Motor Speed and then select ENTER rotate the Control Wheel to select one of the three speeds Fast Medium or Slow then select ENTER to activate the selected speed PRECISION SERIES Pipettor Control Panel ...

Страница 14: ...ered Handle down to engage with the tips b 3 Lift the tips up and out of the rack c and continue processing a c Lift Tips from Rack Pull Lever down Slide in Rack b Set Up and Installation cont To place and pick up tips follow these instructions and refer to the indicated diagram below Note the cautionary guideline below to avoid personal injury CAUTION FOR YOUR SAFETY KEEP BOTH HANDS ON THE LEVERE...

Страница 15: ...anual passes of the pipettor in order to accommodate all 384 wells For the first pass aspirate and or dispense as usual For the second pass slide the microplate to the side with two dots and continue aspirating and or dispensing repeat aspirating and or dispensing for the third and fourth passes at the three and four dot locations respectively An advanced 384 Well Adaptor Accessory is available A ...

Страница 16: ...last 20uL dispense this will continue until all of the aspirated liquid has been dispensed Clears the display screen when an error message appears Saves programs created in the program menu Saves options selected in the options menu such as Motor Speed DISPENSE requires user to enter a liquid dispensing volume uL turn black Control Wheel to select a value between 5 and 250 Adjust volumes slowly us...

Страница 17: ... Normal or Fast Press ENTER once again to save option Pipettor Operating System Select to view the current software version Service Hidden service menu used for calibrating volume and speed Use only when working with a certified Sequence Biotech technician For more information contact Sequence Biotech Products technical Support at 800 767 0665 or email info sequencebiotech com Program Use to creat...

Страница 18: ... over this position ASPIRATING LIqUID INTO THE PIPETTE TIPS Select the ASPIRATE button on the Control Panel rotate the Control Wheel in 1 or 5uL increments to the desired volume The display screen will display Aspirate the desired aspirating volume Current Volume vtracks the volume of liquid aspirated and or dispensed in each of the onetouch tips Using the Levered Handle lower the tips into the ve...

Страница 19: ...sired mixing volume approximately half the volume in the wells and press ENTER Next rotate the Control Wheel to the desired number of mixing cycles 0 25 cycles The display screen will display uL the mixing volume or the amount of liquid to be mixed by each tip Cycles the number of desired mixing cycles Press ENTER to begin the mixing cycles While the pipettor is mixing the display screen will indi...

Страница 20: ... and running programs see additional PROGRAMMING COMMANDS AND ACTIONS in the APPENDIX VIEw EDIT AND SAVE A PROGRAM the Precision Series Pipettor is easily programmed A program containing up to 100 individual steps can be created and saved into the internal flash memory the program may be stored and retrieved by pressing MENU on the Control Panel then choosing Program using the Control Wheel as sho...

Страница 21: ...the command may be modified rotate the black Control Wheel to scroll through the lower level command values Press ENTER again and the curser stops blinking Now rotate the Control Wheel to move the cursor into the next command prompt pressing eNter to select the desired values when prompted repeat the last three steps until the program has been fully edited When done press CANCEL and select Yes or ...

Страница 22: ...ary Alternatively contact Sequence BioTech Technical Support at 603 769 0963 or email service sequencebiotech com and request regular maintenance service or repair To ensure user safety and to avoid warranty invalidation use only factory installed OEM parts and follow proper maintenance and handling instructions The protection afforded by this equipment may be invalidated or impaired if used in a ...

Страница 23: ... procedures are required to ensure the compatibility of the new procedure with the instrument Should an instrument or component that has been used with radioactive or pathogenic material require factory or field service comply with the following procedure to ensure the safety of service personnel Clean the parts to be serviced of all encrusted material and decontaminate them radioactivity must not...

Страница 24: ...wer the 96 channel pipettor Head Assembly by gently pulling down on the Levered Handle until it stops 4 turn the black plastic Locking Thumb Screw on the Chassis Assembly until it completely engages with the c bore in the Chassis Assembly Verify the vertical motion is locked 5 Using the Side Handles lift the instrument to transport to a new location or to place into shipping container 6 to unlock ...

Страница 25: ...rNiNGS retUrNeD iNStrUMeNtS AND or PArtS MUSt ALWAYS iNCLUDe A CoMPLeteD DECONTAMINATION CERTIFICATION FORM note tHe PreCiSioN SerieS PiPettor MUSt Be SHiPPeD iN AN APProVeD PACKAGiNG CoNtAiNer to AVoiD DAMAGe WHiCH MAY iNVALiDAte tHe WArrANtY CoNtAiNerS MAY Be PUrCHASeD For A MiNiMAL CHArGe For ADDitioNAL SerViCe AND SHiPPiNG iNqUirieS CoNtACt SeqUeNCe BioteCH ProDUCtS At 603 769 0963 or eMAiL SE...

Страница 26: ...rint 96 or 384 well 250 uL Pipetting Head Model Dispensing Volume 5 250 uL Dispensing resolution Slow knob rotation 1 uL Fast knob rotation 10 uL Dispensing Precision 25 250 uL 3 5 24 uL 5 Dispensing Accuracy 5 250 uL 3 Speed Slow 13 uL s Speed Normal 62 uL s Speed Fast 78 uL s 20 uL Pipetting Head Model Dispensing Volume 1 0 20 0 uL Dispensing resolution Slow knob rotation 0 1 uL Fast knob rotati...

Страница 27: ...sions Testing FCC Part 15 eN 55011 Class A Immunity Testing eN 61326 1 eN 61000 3 2 Harmonics eN61000 3 3 Flicker Product safety UL CSA eN 61010 1 Valid for all Serial Numbers starting from S N 00100 tHe teCHNiCAL DoCUMeNtAtioN reqUireD to DeMoNStrAte tHAt tHe ProDUCt MeetS reqUireMeNtS HAS BeeN VeriFieD BY BUreAU VeritAS AND iS MAiNtAiNeD BY A rePreSeNtAtiVe oF SeqUeNCe AND iS AVAiLABLe For iNSPe...

Страница 28: ...5 2507l volume Eject Pushes the pipette tips off of the nozzles same effect as pressing the EJECT button on the Control Panel Repeat Next repeats a block of line executions the parameter specifies repetitions how many times the block of line executions are repeated 0 250 the block of lines begins with the line following the Repeat command and ends with the Next command End this is the last line of...

Страница 29: ...Pressing ENTER ends the program and returns the display screen to the upper level menu Below is the same program detailing the steps necessary to enter and create the new program using MENU on the Control Panel the actual screen display follows Press MENU and rotate the Control Wheel to display the following Menu Program Select and press Enter To create a new program or to edit an existing program...

Страница 30: ...n 1 Print Line1 String A new command String appears in line 2 rotate the Control Wheel to String and press ENTER rotate the Control Wheel to select Enter to continue and then press ENTER to save this line into memory 1 Print Line1 Enter to continue to enter the 3rd line 2 wait rotate the Control Wheel back to the number 1 and press ENTER rotate the Control Wheel to select 2 and press ENTER to save...

Страница 31: ...o continue Press ENTER to return back to the main display screen Ready Vol 0 uL standard Pipetting Program example the following program contains multiple steps and is intended to be used as a teaching tool to understand the step based programming method used with the Precision Series Pipettor A program containing up to 100 individual lines can be saved into the internal flash memory Keep in mind ...

Страница 32: ...ertification of the pipettor s accuracy and precision and if necessary recalibration to a standard that is equal to or better than the original manufacturer s specifications Calibration is performed using photometry and includes a 3 3 as left certificate of performance for accuracy and precision at 20 and 250 uL the 3 3 as Left certification indicates that the instrument was calibrated by dispensi...

Страница 33: ...s the calibration described in the Calibration Service Plan with two calibration points and provides all services of the Performance Assurance Service Plan In addition as part of the refurbishing and servicing of the instrument our trained service technician will 1 Clean and inspect the entire instrument and replace or repair any component found to be damaged significantly worn or malfunctioning s...

Страница 34: ... com or your sales representative to schedule warranty work or Service Plan service arrangements We offer a loaner instrument program to supply you with an instrument while your instrument is being serviced Please enquire for details PLAN CHANGES Sequence reserves the right to change plan components and services and plan pricing at any time Changes will not apply to any service plan already ordere...

Страница 35: ...Sequence BioTech offers four unique Service Plans Choose the Plan to best fit your needs Contact sales for more information at info sequencebiotech com 4 4 ...

Страница 36: ...www sequencebiotech com 33 614 Nashua Street Unit 223 Milford NH 03055 4917 P 603 769 0963 F 603 732 0327 ...

Страница 37: ...Important Documents ...

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