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15
General Pipetting set-Up
To ensure safe and accurate liquid handling, follow these simple
instructions to operate the Precision Series Pipettor:
GENERAL PIPETTING SETUP
•
ensure
Levered Handle
is in the upright vertical position.
•
Slide tip rack into secure
Tip Rack Holder
and push completely
back.
•
Using the
Levered Handle
, adjust the horizontal slide to
position the pipettor
Head Assembly
over the tip rack.
•
Using the
Levered Handle
, fully engage 96 pipette tips onto
nozzles using firm downward pressure. raise the
Levered
Handle
to the highest position.
•
Set the tip depth for the reservoir using the
Height Adjustment
Posts
. refer to vertical
Height Adjustment
in the Set-Up and
installation section. Set the tip depth for the plate/block
loading position using the
Height Adjustment Posts
.
•
Place the desired reservoir into position two.
•
Move the pipettor
Head Assembly
to the second position.
the instrument will self-center directly over this position.
ASPIRATING LIqUID INTO THE PIPETTE TIPS
•
Select the
ASPIRATE
button on the
Control Panel
. rotate the
Control Wheel (in 1 or 5uL increments) to the desired volume.
The display screen will display:
“Aspirate”
—the desired aspirating volume
“Current Volume”
vtracks the volume of liquid aspirated and/or
dispensed in each of the onetouch tips
•
Using the
Levered Handle
, lower the tips into the vessel.
the tips automatically stop at a depth defined by the
Height
Adjustment Post
setting. tips should immerse into liquid at
a depth of 2mm.
•
Press the
ENTER
button on the
Control Panel
to aspirate the
selected volume.
“Current Volume” appears on the display screen to indicate
the selected volume.
•
once the pipette tips contain the aspirated sample volume,
raise the pipettor
Head Assembly
to the highest position using
the
Levered Handle
.
•
Slowly slide the pipettor
Head Assembly
into the notched guide
at position three so that it “seats” over the target plate.
•
Slowly and carefully lower the pipettor
Head Assembly
so
that the ends of the tips do not touch the bottom of the wells
(the
Height Adjustment Post
settings prevent bottoming out).
Basic Operation
cont.