Irys® User Guide
07/10/2015
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For Research Use Only. Not Intended For Diagnostic Purposes.
Chapter 1 Overview
1.1 Introduction
Complex genomes are composed of multiploid chromosomal DNA with each individual chromosome ranging from
hundreds of thousands to hundreds of millions of base pairs in length. Once extracted from cells, these long
biopolymer molecules form tight ball-like coils that are difficult to image and analyze. Irys uses a patented method
of DNA linearization to unravel the DNA and then image it in the NanoChannel Array, allowing the structure of the
genome to be imaged and then analyzed at the molecular level.
The Irys System is a groundbreaking technology for performing single molecule and whole genome mapping and
analysis in a nanoscale fluidic environment designed to enable the identification of biological samples on a
molecule-by-molecule basis. It is suitable for a variety of applications including whole-genome sequence scaffolding
and structural variation (SV) region mapping, identification, and quantitation. The Irys System uses a
straightforward and flexible process, and consists of five main steps:
1. Sample preparation
— Performed using the IrysPrep sample preparation kit for your specific application.
2. Chip preparation
— Performed on the Irys.
3. Molecule imaging
— Performed on the Irys.
4. Data viewing and genome analysis
—Performed using IrysView.
5. Data analysis
— Data analysis starts with AutoDetect, the image detection software, which is performed off-
instrument. Further data analysis is performed off-instrument using IrysView and IrysSolve software
applications.
What's New!
• New version recipes enable 2-color.
• Version M3 and M3x are new base recipes.
• M3 and M3x Generate Optimized recipes are new user-defined configurations to improve workflow for
throughput.
• Graphical user interface enhancements include additional options for file saving in the Scan Flowcell
window.
Audience and Intended Use
This guide contains information about the Irys system. It provides an overview of instrument components and
software features, and full instructions for performing a run.
This guide is for laboratory personnel and other individuals responsible for:
• Operating the instrument
• Performing runs
• Performing instrument and component maintenance
• Training personnel on the instrument
Irys is intended for RESEARCH USE ONLY (RUO) and is not for use in diagnostic procedures.
1.2 Irys Overview
Irys Description
Irys is an epi-fluorescent imager capable of detecting single DNA molecules in submicron channels etched into a
silicon substrate. The system is comprised of optical and mechanical components that are designed and optimized
to provide imaging capabilities for single molecule analysis.
