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Learning to Create Methods for the Biomek® FX

2-13

Biomek® FX and FX-P Laboratory Automation Workstations Quick-Start Guide

2.5

Configuring a Combine Step for Span-8 Pod

If your instrument does not have a Span-8 Pod, skip this section and continue to the 
next.

The 

Combine

 step transfers liquid from one or more sources to a single destination. 

You are going to insert and configure a 

Combine

 step using a Span-8 Pod into the 

method to transfer liquid from the 

Source

 96-well microplate to the upper left 

quadrant of the 

Destination

 384-well microplate using the 

Combine

 step. 

Note:  

For information on the extensive selections available for configuring the 

Transfer

 step, refer to the 

Biomek Software User’s Manual, 

Chapter 16.3, 

Configuring Transfer and Combine Steps

.

2.5.1

Inserting a Combine Step

To insert a 

Combine

 step into your method:

Drag and drop a 

Combine

 step from the Step Palette into the Method View just 

before the 

Finish

 step. Notice that the Current Deck Display shows the current 

position of all labware on the deck (Figure 2-11).

You’ll complete three sections to configure the 

Combine

 step for this method:

•

Section 2.4.2, 

Configuring Tip Handling

•

Section 2.4.3, 

Configuring Source Labware

•

Section 2.4.4, 

Configuring Destination Labware

Figure 2-11.  Combine step inserted into the Method View

A 

Combine

 step loads 

tips, aspirates and 
dispenses liquid, and 
unloads tips. This 
eliminates the need to 
insert four separate steps 
to complete a liquid 
transfer. However, there 
are steps in Biomek 
Software that allow these 
steps to be performed 
individually with a 
Span-8 Pod on the 
Span-8 Step Palette (refer 
to the 

Biomek Software 

User’s Manual, 

Chapter 

19, 

Using the Span-8 Step 

Palette

).

Combine Step

Current Deck Display

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Содержание BiomekFX

Страница 1: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

Страница 2: ...821 Copyright 2009 Beckman Coulter Inc Biomek FX and FXP Laboratory Automation Workstations Quick Start Guide Beckman Coulter PN 987838 Revision AE December 2009 Artisan Technology Group Quality Instr...

Страница 3: ...he procedures specified in this manual regardless of foreseeability or the form of action whether in contract tort including negligence breach of warranty strict liability or otherwise and including b...

Страница 4: ...quired by the Company be returned to the factory transportation charges prepaid and will be returned to Purchaser with transportation charges collect unless the product is found to be defective in whi...

Страница 5: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 6: ...2 Launching the Software 1 7 1 4 3 Homing All Axes of the Biomek FX Pod 1 8 2 Learning to Create Methods for the Biomek FX 2 1 Overview 2 1 2 2 Creating a New Method 2 2 2 3 Configuring the Instrument...

Страница 7: ...Editors While Creating Methods 3 1 Overview 3 1 3 2 General Description of Editors 3 2 3 2 1 Project Menu 3 3 3 2 2 Instrument Menu 3 4 3 3 Creating New Labware in the Labware Type Editor 3 5 3 4 Crea...

Страница 8: ...re with your Biomek FX This quick start guide includes Introducing the Biomek FX Laboratory Automation Workstation this chapter a general hardware overview on the Biomek FX Laboratory Automation Works...

Страница 9: ...tion links are provided throughout this guide to specific sections in the Biomek FX and FX P Laboratory Automation Workstations User s Manual the Biomek Software User s Manual and the ALPs User s Manu...

Страница 10: ...ting components such as heads ALPs and third party devices Regardless of pod configuration or operating components your Biomek FX is controlled through Biomek Software A light curtain and other protec...

Страница 11: ...terchangeable platform structures that are installed on the Biomek FX deck to allow automated assays to be performed ALPs are either active or passive Passive ALPs hold labware in place on the deck or...

Страница 12: ...s do not degrade light curtain operation however it is advisable to test the impact of all lab attire on light curtain sensitivity before operating the Biomek FX The Biomek FX is equipped with protect...

Страница 13: ...nfiguration options appear in the Step Configuration Area refer to Chapter 2 Learning to Create Methods for the Biomek FX The method is customized to the desired level of precise control via the edito...

Страница 14: ...f you are logging into Biomek Software for the first time you must enter a new password Remember your new password you will need to enter it each time you access Biomek Software Figure 1 2 Biomek Soft...

Страница 15: ...hich all subsequent moves are determined To home all axes 1 From the Instrument menu choose Home All Axes A Warning appears 2 Make sure that all warnings are addressed and choose OK The pod is homed a...

Страница 16: ...s or if you need additional information along the way use the links provided to access the suite of user s manuals provided during installation These manuals include Biomek Software User s Manual Biom...

Страница 17: ...into the Method View A new method is created and assigned the default name Method1 New Figure 2 1 Figure 2 1 Biomek Software main editor for dual pod Biomek FX instrument If using Accounts Permission...

Страница 18: ...ear the Biomek FX deck clear the devices on the deck unload tips from the pod and clear all global variables Refer to the Biomek Software User s Manual Chapter 13 2 2 Configuring the Finish Step for i...

Страница 19: ...what labware and ALPs are on the deck You ll learn more about the Deck Editor in the next chapter refer to Section 3 5 Creating a New Deck Figure 2 2 Instrument Setup step in the Method View When buil...

Страница 20: ...isplay during setup drag and drop the labware to the Clear trash icon Figure 2 3 Instrument Setup step with labware added If you want to use a labware type that does not appear in the availablelabware...

Страница 21: ...e on P5 and name it Dest1 in Labware Properties 10 In Labware contains select Known 11 In volume enter 50 Labware Properties for the labware on P5 should look like Figure 2 5 12 In liquid type select...

Страница 22: ...the current state of the pod for example if the pod already has tips loaded Your Instrument Setup step is now configured and the main editor should look like Figure 2 6 Note To learn more about the In...

Страница 23: ...om the Step Palette into the Method View just below the Instrument Setup step Notice that the Current Deck Display shows the current position of all labware on the deck Figure 2 7 You ll complete thre...

Страница 24: ...ces 6 Uncheck Change tips between destinations 7 Your tips are configured for your liquid transfer so click the up arrow next to Tip Handling to collapse the configuration This allows more room for th...

Страница 25: ...hand up and down until the depth is as close to 1 00 mm from bottom as you can get Then adjust the height precisely to 1 00 mm using the following note This will be your aspirate height for this tran...

Страница 26: ...area 3 The Volume field is highlighted which allows you to designate the amount of liquid to be dispensed For this tutorial you re transferring 100 L so type 100 into the Volume field Note The volume...

Страница 27: ...skip to Section 2 6 Configuring a Move Labware Step to learn how to move labware using the gripper on the Multichannel Pod Figure 2 10 Transfer step with Destination configured Note The method view ch...

Страница 28: ...a Combine step into your method Drag and drop a Combine step from the Step Palette into the Method View just before the Finish step Notice that the Current Deck Display shows the current position of a...

Страница 29: ...ed 3 Click on the AP96_20 L_LLS tip box in the Current Deck Display to load LLS tips as part of the Combine step 4 Make sure unload them is selected in the next field 5 Check Change tips between trans...

Страница 30: ...h selected well 4 Leave the Auto Select checkbox selected to use the technique selected by Biomek Software 5 Right click on the large tip illustration next to the microplate graphic in the configurati...

Страница 31: ...an Coulter Inc The source labware configuration is complete and the main editor now looks like Figure 2 13 Figure 2 13 Combine step with source labware configured Artisan Technology Group Quality Inst...

Страница 32: ...he source labware configuration is now replaced with a brief sentence summary of the setup If you want to reopen this source configuration for any reason click anywhere in the collapsed configuration...

Страница 33: ...15 Figure 2 15 Combine step with destination labware configured The Combine step configuration is now complete In the next section you will configure a Move Labware step to move labware using the grip...

Страница 34: ...gure a Move Labware step to move your Dest1 labware at position P5 to another deck position 1 Insert a Move Labware step into the Method View 2 In Move labware from select P5 from the drop down menu 3...

Страница 35: ...rmation on configuring the Finish step 2 7 1 Handling Configuration Errors If there is a step configuration error in your method the step with the error is highlighted in red Figure 2 17 and the ETC i...

Страница 36: ...t items such as labware class definitions or techniques changes after the method is saved when the method is opened next the latest definitions are used Refer to the Biomek Software User s Manual Chap...

Страница 37: ...mek FX Beckman Coulter Inc Figure 2 19 Quick Start method saved and named Move on to Section 2 9 Running a Method to run your saved Quick Start method Artisan Technology Group Quality Instrumentation...

Страница 38: ...e 2 21 from the Instrument menu choose Hardware Setup Biomek Hardware Setup appears Figure 2 20 Hardware Setup 2 In Port select Simulate 3 Choose Accept to save changes and close Hardware Setup Biomek...

Страница 39: ...configuration area is replaced with a three dimensional model of your Biomek FX Figure 2 21 The deck confirmation prompt Figure 2 22 appears on top of the main editor Figure 2 21 Biomek FX simulator...

Страница 40: ...the Method View as steps are highlighted in green as they are executed Note If you are running this method on your instrument instead of the simulator make sure that the physical deck layout matches t...

Страница 41: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 42: ...s The editors are accessed from the Project or Instrument menus located on the toolbar of the Biomek main editor Figure 3 1 Read the information in this chapter and complete the tutorial steps provide...

Страница 43: ...u will work with three of the more commonly used editors and Manual Control in this quick start guide If using Accounts Permissions You must have Develop Projects and Setup Instrument permissions to a...

Страница 44: ...d Modifying Tip and Labware Types Well Pattern Editor allows you to create and store patterns for hitting specific wells refer to the Biomek Software User s Manual Chapter 12 Creating Well Patterns No...

Страница 45: ...o use the device it must be configured through the Device Editor refer to the Biomek Software User s Manual Chapter 23 4 1 Configuring Devices Using the Device Editor Manual Control allows you to cont...

Страница 46: ...ifying Tip and Labware Types Note Do not run a method outside of the simulator using the labware you create here the specifications may not be accurate for the physical piece of labware you use in thi...

Страница 47: ...he4n edit the copy of the labware type rather than just editing the predefined labware to meet the new labware specifications 3 Enter NewLabwareQuickStart and choose OK The copy of BCFlat96 is created...

Страница 48: ...e 3 5 NewLabwareQuickStart Note Each piece of labware in Labware Types has its own mini editor similar to Figure 3 5 to allow specifications to be revised and saved 5 In Height enter 1 46 and choose S...

Страница 49: ...ay be used or copied to create new patterns The patterns created and stored in Well Patterns are accessible in the Transfer and Combine steps using the Span 8 Pod To create a new well pattern based on...

Страница 50: ...tions Quick Start Guide 2 Choose New New Pattern appears Figure 3 7 Figure 3 7 New Pattern 3 Highlight NewLabwareQuickStart and choose OK Figure 3 8 appears Figure 3 8 Well Patterns based on Pattern 9...

Страница 51: ...lect the wells for the new pattern so it looks like Figure 3 9 Figure 3 9 Wells chosen for the new pattern 5 Choose Rename to rename the new pattern Rename Pattern appears Figure 3 10 Figure 3 10 Rena...

Страница 52: ...reQuickStart and choose OK Figure 3 11 appears Figure 3 11 New pattern renamed 7 Choose Save to save LabwareQuickStart in Well Patterns Note Refer to the Biomek Software User s Manual Chapter 12 Creat...

Страница 53: ...is deck appears Figure 3 13 Figure 3 13 Choose a name for this deck 3 Enter TwoWashDeck and choose OK TwoWashDeck appears 4 Click and hold the mouse button on Span8WashLeft from the ALP Types List Loc...

Страница 54: ...is now the default deck and is available in the Instrument Setup step Figure 3 14 TwoWashDeck with second Span 8Wash added 6 Select Open Deck Select a Deck appears Figure 3 15 Figure 3 15 Select a De...

Страница 55: ...also for controlling individual ALPs and Device Controller outside a method refer to the ALPs User s Manual Using the following instructions you ll use Manual Control to move the pod to a different po...

Страница 56: ...t Pod1 or Pod2 Advanced Manual Control for the selected Pod appears Figure 3 18 Figure 3 17 Pod1 highlighted in Advanced Controls Figure 3 18 Advanced Manual Control for a Multichannel Note Advanced M...

Страница 57: ...o position P5 on the deck 6 You can click on additional positions to move the pod to that position When you are finished moving the pod with Manual Control choose Exit to close Manual Control 7 From t...

Страница 58: ...e You ve completed a small method steps using the editors steps to manually control the Biomek FX If you ve used the links to other manuals you ve also learned where to find hardware information on th...

Страница 59: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 60: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

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