Instruction for Use HISTO TYPE Rainbow QS6
Version: 02/2021
Page 7 of 16
Please note:
When pipetting into the PCR wells it is important not to allow the pipette tip to contact
the dried mix (dyed blue) in the bottom of the well. It is advisable to pipette to the side of the well to
allow the 10 µl to mix by gravity with the dried mix (see Figure 1).
Figure 1
Figure 2:
HISTO TYPE Rainbow QS6 plate. In wells A1 to G12 are the dried specific mixes (blue coloured). In well H12
there is the dried NTC (also blue).
Seal the PCR plate with the supplied qPCR seal and briefly spin the liquid down. Make sure that the
plate is
completely sealed
, particularly at the edge of the plate. Make sure that the liquid has
contacted the dried mix and there are
no bubbles
or air gaps in the reaction wells. If bubbles
appear, gently tap the tubes on the laboratory bench to remove them, or preferably careful spin it
down (10 sec).
Place the sealed PCR plate in the QS6 instrument. Please use the correct plate holder and
orientation as per the notes below.
Note:
Please make sure that you place the reaction plate into the cycler in the correct orientation
as shown in Figure 2 above where the NTC well is in the bottom right corner, position H12.
Note:
For the HISTO TYPE Rainbow plates a special plate holder for the
QuantStudio™ 6 Flex
System is required, which is included in the first starter package (REF 726321). For more
information please contact BAG Diagnostics.
