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Instruction for Use HISTO TYPE Rainbow QS6 

Version: 02/2021 

Page 9 of 16 

 

 

6.5 

Export of results from 

QuantStudio™ 6 Flex System 

 

After  the  PCR  run  is  complete  there  is  no  requirement  for  analysis  in  the  Applied  Biosystems 
software.  For  analysis  in  PlexTyper

®

  the  QS6  .eds  file  must  be opened and an excel file exported 

using either on the machine software or on separate QS6 software.  
 
 
Export the raw data in excel format with the following steps. 
 

1)  Select the .eds file, double click on a .eds file. If the QuantStudio RealTime PCR software is 

installed the software will automatically open the .eds file. Navigate to the export button and 
select export. 
 

 

 
 

2)  In  QS6  software  (with  reference  to  image  below)  (A)  select  the  folder  location  where  you 

want the export to go, select open folder to save. (B) If required rename the file by prefixing 
with the PT appropriate run ID from entering the sample in PlexTyper

®

  prior to running test. 

(C) if not selected, select excel format, (D) select export 

 

Содержание HISTO TYPE Rainbow QS6

Страница 1: ...ample information into PlexTyper Software 5 6 4 Amplification 5 6 5 Export of results from QuantStudio 6 Flex System 9 6 6 Evaluation and interpretation of the results 10 6 7 Import of the results fil...

Страница 2: ...s amplified in a real time PCR with sequence specific primers SSP The primers were specially developed for the selective amplification of segments of specific HLA alleles or allele groups The amplicon...

Страница 3: ...2 Real time PCR Cycler validated cycler see 4 3 Plate holder QS6 REF 726321 Variable pipettes 0 5 1000 l and pipette tips Application spatula for qPCR Seal Molecular grade DNAse free water Suitable pl...

Страница 4: ...amplification detection use two separate rooms if possible Use devices and other materials only at the respective workplaces and do not exchange them 6 2 DNA Isolation The specimen material for the i...

Страница 5: ...entity This Run ID should be used when setting up the file identity in the PCR machine If the PCR excel export file is not pre labelled with the RUN ID the file can be manually prefixed with the corre...

Страница 6: ...te the test DNA with other concentrations For DNA of different stock concentrations use the dilution table below to adjust the DNA to working concentration Add 8 l Molecular grade water and 2 l Plex M...

Страница 7: ...ely sealed particularly at the edge of the plate Make sure that the liquid has contacted the dried mix and there are no bubbles or air gaps in the reaction wells If bubbles appear gently tap the tubes...

Страница 8: ...Flex System Block type Fast 96 Well 0 1mL Experiment type Comparative Ct Ct Reagent type TaqMan Reagents Run properties Standard Define Targets Please note Custom dye instrument calibration must be p...

Страница 9: ...rt the raw data in excel format with the following steps 1 Select the eds file double click on a eds file If the QuantStudio RealTime PCR software is installed the software will automatically open the...

Страница 10: ...s required for the evaluation are available from the BAG Diagnostics download server www service bag diagnostics com Please make a note of the lot number of the kit The interpretation kit files are pr...

Страница 11: ...s possible contamination by the PlexTyper software and a warning message is generated Amplification signals above Cq 36 in the NTC are regarded as PCR artefacts and are disregarded If PCR contaminatio...

Страница 12: ...No results at an HLA locus 7 WARNINGS AND DISPOSAL INSTRUCTIONS HISTO TYPE Rainbow QS6 is designed for in vitro diagnostic use The kit should only be used by specially trained qualified personnel All...

Страница 13: ...t is checked for each lot with control specimens with known HLA alleles The kit determines the HLA Loci A B C DRB1 DRB3 DRB4 DRB5 DQA1 DQB1 DPA1 and DPB1 8 1 SPECIFIC PERFORMANCE CHARACTERISTICS 8 1 1...

Страница 14: ...n to avoid contamination of kit reagents and other laboratory materials with amplicons or DNA The performance of a negative control without DNA in well H12 is strongly recommended No fluorescence sign...

Страница 15: ...ll of the tube Careful pipetting Spin down PCR plate User error Ensure all wells receive the required volume of reagents Evaporation of the reagents due to incorrect closing of the PCR tubes Make sure...

Страница 16: ...ue number RTU Ready to use 14 LITERATURE 1 Mack S J et al 2013 Tissue Antigens 81 194 203 2 Beutler E et al 1990 BioTechniques 9 166 15 REVIEW HISTORY Version 01 2021 Issue 2021 04 Initial version Ver...

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