Instruction for Use HISTO TYPE Rainbow QS6
Version: 02/2021
Page 6 of 16
DNA concentration 10-20 ng/µl
•
Add
805 µl
molecular grade water to the vial with 230 µl Plex Mix and mix (vortex briefly 1-3
sec).
•
After mixing, pipette
10 µl
of the mix into the NTC well (well 96; position H12 -see also
Figure 1 and 2).
•
Then pipette
115 µl
DNA into the vial with the remaining Plex Mix-water-mixture and mix
(vortex briefly 1
– 3 sec).
•
Distribute 10 µl of the DNA/Plex Mix/water solution into each of the wells 1-95 of the HISTO
TYPE Rainbow QS6 plate (please note figure 1 and 2). The NTC well (well 96; position H12)
must not be filled with the DNA mix as this will cause the NTC to be positive and can
invalidate the test!
DNA with other concentrations
For DNA of different stock concentrations use the dilution table below to adjust the DNA to working
concentration.
•
Add
8 µl
Molecular grade water and
2 µl
Plex Mix to the NTC (well H12). The NTC well (well
96; position H12) must not be filled with the DNA mix!
•
Pipette the DNA and the molecular grade water into the remaining 228 µl Plex Mix according
to the following table and mix (vortex briefly 1-3 sec)
Depending on the concentration of DNA, pipette the applicable volumes in the table below to the
228 µl Plex Mix remaining in the vial after setting up the NTC:
Concentration of the DNA [ng/µl]
Molecular grade water [µl]
DNA volume [µl]
2
342
570
5
684
228
50
889
23
80
898
14
100
901
11
150
904
8
200
906
6
250
907
5
300
908
4
500
910
2
•
Distribute
10 µl
of the DNA-Plex Mix-water solution into each of the wells 1-95 of the HISTO
TYPE Rainbow QS6 plate.
