Operation
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21
3. In
the
Filter Tool
program, click the checkbox for the filter position you are
adding. The Filter Wavelength dialog box will appear (Figure 7).
Figure 7.
Mid-point wavelength editor.
4. Enter the midpoint of the wavelength range that is transmitted by the filter.
For a band-pass filter, a 530DF30 filter, for example, transmits a 30 nm
band of light centered at 530 nm (
i.e.,
it transmits 515 – 545 nm); therefore
the midpoint of this filter is entered as “530”. For a long-pass or a ND
filter, enter the peak emission of the dye you will use with that filter.
5. In GenePix Pro you can display either the filter’s
Formal name
(
e.g.,
“530DF30”) or an
Alternate name
(
e.g.,
“Alexa 488” or “Blue Filter”).
Enter both names here so that each user can choose their preferred
nomenclature in GenePix Pro. Under
Formal name
, enter the midpoint of
the wavelength range that is transmitted by the filter followed by the total
wavelength range. For example, a filter that is centered at 530 nm and
transmits a 30 nm band of light (
i.e.,
it transmits 515 – 545 nm) will be
named “530DF30”. For a long-pass or a ND filter, enter the peak emission
of the dye you will use with that filter. Under
Alternate name
, enter the
name of the dye that this filter set will be used for or a name identifying the
color dye it will be used for.
6. After you have entered this information for each new filter, click
Save
.
7. Close
the
Filter Tool
program.
Note:
When you open GenePix Pro, the new filters are listed in the Hardware
Settings dialog box.
Chapter 3
