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Aquaprobe
®
Lite Instruction Manual
10401-00976 Rev F
Another particular problem when trying to measure very low
concentrations is air in the form of both visible and
microscopic bubbles. These act like tiny prisms and can
refract and reflect both the excitation light and the return
signal being measured.
The photograph to the right was taken in a calibration tube
after fresh water was poured in. The bubbles are clearly
visible in the light beam.
2.4. Top Tips for successful measurements using optical electrodes
Always keep the measurement chamber and electrode lenses clean.
Always fit the sleeve and end cap during both calibration and measurement.
Always allow the readings to settle completely during both calibration and
measurement.
Always try to eliminate air bubbles by agitating the Probe after insertion both during
calibration and measurement. On the AP-7000, activate the self-cleaning mechanism to
clear bubbles.
Always calibrate and zero the electrode as close to your sample temperature as
possible. This is especially important with the Ref-Oil electrode.
Always zero the electrode just prior to use in clean water (bottled still mineral water is
ideal). This is also especially important with the Ref-Oil electrode.
2.5. About Fluorescent Measurement
All Optical Electrodes, with the exception of the Turbidity Electrode, employ fluorescent
measurement techniques. Interference from microbiological species and compounds which
fluoresce at similar wavelengths and differences in fluorescence caused by temperature,
ambient light and turbidity can all cause inaccuracies.
Fluorescence measurement is ideal for researchers who are interested in detecting the
presence or absence of a specific substance in reasonable concentrations and measuring
relative fluorescence changes that can be used as an indication of increasing or decreasing
concentrations.
Fluorescence measurement techniques are not ideal for quantitative measurement and it is
therefore impossible to specify an absolute accuracy.
In order to obtain accurate results, data obtained with a fluorescent electrode in the
field must be post-calibrated with data from standard laboratory analysis of grab
samples acquired during the study.
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Ltd.
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