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Aquaprobe
®
Lite Instruction Manual
10401-00976 Rev F
11.10. 2000-CDOM/FDOM Chromophoric (Fluorescent) Dissolved Organic Matter
Fluorescent) Dissolved Organic Matter can be measured by the AP-Lite using the optional
2000-CDOM optical electrode.
11.10.1.
Principle of Operation
The 2000-CDOM optical electrode is a submersible, fixed response fluorometer, which
provides excitation at 365nm (UV) and detects any resultant fluorescence between 450nm
and 520nm.
The electrode induces the dissolved organic matter to fluoresce, then measures the longer
wavelength light which is emitted as a result of the fluorescence process.
➔
During operation, the CDOM Electrode emits high intensity ultraviolet (UV)
light, which is harmful to skin and eyes and may cause cancer. Avoid
exposure to UV light when the Electrode is in operational.
➔
Precautions must be taken to avoid looking directly at the Electrode without
the use of UV light protective glasses.
➔
Do not look directly at the lenses on the front face of the Electrode when it is
operational.
➔
Ensure the warning label supplied with the Electrode is attached to the
Aquaprobe
®
.
11.10.2.
Limitations of Use
Determination of CDOM in the field using fluorescence measurement techniques will never
be as accurate as measurements made in a lab using traditional techniques.
Factors adversely affecting accuracy include:
•
Interference from compounds which fluoresce at similar wavelengths.
•
Differences in the fluorescent response caused by temperature.
•
Differences in the fluorescent response caused by ambient light.
•
Interference caused by turbidity.
Fluorescence measurement techniques are ideal for researchers who are interested in
detecting the presence or absence of a specific substance and measuring relative
fluorescence changes that can be used as an indication of increasing or decreasing
concentrations.
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®
Ltd.
www.aquaread.com
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