Applied Biosystems GeneAmp PCR System 9600, Руководство пользователя

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CYCL: The Cycle Program
A CYCL program contains the thermal ramp and hold segment
patterns for PCR cycling. It contains up to nine setpoints. Each
setpoint comprises a ramp segment and a hold segment for the
specific target temperature. The pattern can be repeated up to 99
times. A complete cycle for a common profile requires about
three or four minutes.
Typically, there are two or three setpoints in a CYCL program.
The first setpoint is commonly
used for DNA denaturation or
melting. For example, the setpoint temperature can be set to 94
degrees C for about 10 to 20 seconds, when double strands of
DNA are separated into single strands.
The second setpoint is commonly used for primer annealing. For
example, the setpoint temperature can be lowered to between 45
and 55 degrees C for typically not less than 15 seconds, when a
unique pair of primers bind or anneal to the start and end points
of the target sequence.
For the third setpoint, the primers are extended. For example,
DNA can be polymerized at approximately 72 degrees C for a
time interval of typically not less than 15 seconds.
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