Antec ALEXYS OR 100, Руководство пользователя, Страница: 40 / 46

40 User manual OR 100 /110, ed. 5
is switched off and the pressure has dropped to atmospheric pres-
sure.
When the ALEXYS LC-EC system is not used for a longer period of time
(weeks) follow the system shutdown procedure.
Precautions and system shutdown
The ALEXYS system has been developed for continuous operation. For
maximum stability it is advised to leave the system ON continuously. If pre-
ferred, the flow cell may be switched OFF at night. However, precautions
should be taken when:
•
system is not in use for longer period of time (more than a few days)
•
using mobile phase buffers at pH < 2 or pH > 9
•
using corrosive mobile phase
1. Always switch off flow cell when changing to solvents with-
out electrolytes.
2. First read column manufacturer recommendations. Take out
column in case of solvent incompatibility.
3. Start always with flushing LC system with water when switch-
ing solvents.
Corrosive solvent precautions
When the system is not in use overnight or weekends switch off the cell
and read column manufacturer recommendations. Flush with an appropri-
ate solvent and only take out column in case of solvent incompatibility.
Flush system with pure water and set the HPLC pump to a low flow rate.
Next working day change water for mobile phase and continue operation.
Before switching on the cell make sure mobile phase has been flushed
throughout the system.
Corrosive solvents are: high concentrations of sodium hydroxide (carbohy-
drate analysis) or other buffers of pH>9 or pH < 2.
System shutdown
When not in use for a number of days flush the OR 100/110 first with water
then with iso-propanol or 50/50 water/methanol. Store column and flow cell
according to manufacturer recommendations. Then turn off all ALEXYS
system components. Turn off the OR 100. Disconnect all tubing from the