Basic information
6
1.2
Purpose
The qTOWER³ auto is a thermal cycler developed for real-time PCR experiments which
amplify DNA by way of the polymerase chain reaction (PCR), while using fluorescence
spectroscopy for the highly sensitive detection of target sequences. The signal from the
fluorescent dyes excited by a light source correlates, in terms of quantity, with the PCR
product and may be shown in real-time.
Thanks to a modular design that has been specially adapted to meet the requirements
of automation technology, the device can be used for all relevant real-time PCR applica-
tions, for example for the detection of pathogens, gene expression analysis, SNP geno-
typing or mutation detection, as well as for experimental studies with increased sample
throughput.
Optimized for the area of gene expression and genotyping, the real-time PCR cycler al-
lows for multiplex analyses depending on the fluorescence properties of the dyes and
sensors, as well as the available filter modules – proof of several target sequences in a
single PCR reaction. Analytik Jena offers a number of color or FRET modules here, up to
six of which can be installed simultaneously in the device.
In addition to the optical components for the detection of real-time signals, the amplifi-
cation of the target sequences plays a central role. With Peltier technology and the use
of high-quality block materials, the qTOWER
3
auto boasts a superb thermal homogene-
ity, maximum speed and accuracy.
The qTOWER³ auto is fully controlled from the PC using the qPCRsoft auto application
software. The control software is simple and intuitive, and guides the user on a step-by-
step basis through the programming, data acquisition and analysis menu items. Further-
more, the software offers a wide spectrum of free analysis tools which allow for the
quick and reliable evaluation of comprehensive analyses:
Absolute and relative quantification
ΔΔCt
– Analyses with/without PCR efficiency
DNA melting curves
SNP genotyping
End point analyses
Further information on the various qPCRsoft auto software functions can be found in
the software user manual.
We recommend the following scientific publication for an intensive introduction into the
real-time PCR analysis techniques and applications:
LOGAN, Julie; EDWARDS, Kristin; SAUNDERS, Nick (ed.):
Real-Time PCR – Current Tech-
nology and Application
. Norfolk UK: Caister Academic Press, 2009
