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42 Agilent 1100 Series Fraction Collectors User’s Guide
1 Configuration and Operation of the Fraction Collector
Optimizing fraction collection
Optimizing fraction collection
Time-based
fraction
collection
• Time slices must have a length of at least 0.05 min.
• Set # of Fractions such that length of resulting fractions is at least 0.05 min.
Peak-based
fraction
collection
• Set threshold and slope values such that length of fractions is at least 0.05
min.
• Unresolved peaks can be separated using appropriate threshold and slope
values. If two unresolved peaks are to be collected as one fraction, collect
based on threshold only.
• If the baseline of the chromatogram is below or above 0 mAU, this offset is
not accounted for when triggering peaks using a threshold value. The
threshold value is always added to 0 mAU.