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5
Agilent DNA 7500 and DNA 12000 Assay Protocol
Loading the Gel-Dye Mix
Loading the Gel-Dye Mix
1
Allow the gel-dye mix to equilibrate to room
temperature for 30 minutes before use. Protect the
gel-dye mix from light during this time.
2
Take a new DNA chip out of its sealed bag and place
the chip on the chip priming station.
3
Pipette 9.0 μl of the gel-dye mix at the bottom of the
well marked and dispense the gel-dye mix.
4
Set the timer to 30 seconds, make sure that the plunger is positioned at
1 ml and then close the chip priming station. The lock of the latch will
click when the chip priming station is closed correctly.
N O T E
Before loading the gel-dye mix, make sure that the base plate of the chip priming station is
in position (C) and the adjustable clip is set to the highest position. Refer to
“Setting up
the Chip Priming Station”
on page 8 for details.
OJHOG\H
N O T E
When pipetting the gel-dye mix, make sure not to draw up particles that may sit at the
bottom of the gel-dye mix vial. Insert the tip of the pipette to the bottom of the chip well
when dispensing. This prevents a large air bubble forming under the gel-dye mix. Placing
the pipette at the edge of the well may lead to poor results.
