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Agilent DNA 7500 and DNA 12000 Assay Protocol
Cleaning Electrodes after a Chip Run
Cleaning Electrodes after a Chip Run
When the assay is complete,
immediately
remove the used chip from the
Agilent 2100 Bioanalyzer and dispose of it according to good laboratory
practice. Then perform the following procedure to ensure that the electrodes
are clean (i.e. no residues are left over from the previous assay).
1
Slowly fill one of the wells of the electrode cleaner with 350 μl deionized
analysis-grade water.
2
Open the lid and place the electrode cleaner in the Agilent 2100
Bioanalyzer.
3
Close the lid and leave it closed for about 10 seconds.
4
Open the lid and remove the electrode cleaner.
5
Wait another 10 seconds to allow the water on the electrodes to evaporate
before closing the lid.
N O T E
Use a new electrode cleaner with each new kit.
C A U T I O N
Leak currents between electrodes
Liquid spill may cause leak currents between the electrodes.
➔
Never fill too much water in the electrode cleaner.
N O T E
Replace the used electrode cleaner with each new kit.
N O T E
When switching between different assays, a more thorough cleaning may be required.
For more details please refer to the "Maintenance and Troubleshooting Guide" which
is part of the Online Help of the 2100 Expert software.
