64
Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference
3
Optimization of the Agilent 1260 Infinity Quaternary LC VL
Optimizing Detection with DAD
Optimization Overview
Table 10
Optimization Overview
Parameter
Impact
1
Selection of flow cell
•
peak resolution versus sensitivity
•
Choose flow cell according to used column, see
2
Connection of flow cell
•
chromatographic resolution
•
For flow rates from 0.5 ml/min connect column using the zero-dead-volume
fittings of the detector.
•
For small column i.d. (e.g 1 mm) the inlet capillary of the micro flow cell can
be connected directly to the column.
3
Setting the peak width (response time)
•
peak resolution versus sensitivity
versus disk space
•
Use peak width according
on page 65 as starting
point.
•
Set the peak-width close to the width of a narrow peak of interest in your
chromatogram.
4
Setting wavelength and bandwidth
•
Sample wavelength:
•
Never miss a peak by the use of a browser wavelength like 250 nm with
100 nm bandwidth.
•
Select specific wavelength with reduced bandwidth if you need
selectivity, e.g. 250,10 nm and 360,100 nm as reference wavelength.
•
Set the sample wavelength to a peak or valley in the spectrum to get
best linearity for high concentrations.
•
sensitivity versus selectivity
•
sensitivity versus linearity
•
Reference wavelength:
•
Select the reference wavelength with broad bandwidth (30...100 nm)
wavelength range where your analytes have little or no absorbance (e.g.
sample at 254 nm, reference at 320 nm).
•
baseline drift due to RI effects.
Содержание 1260 Infinity Quaternary LC VL
Страница 1: ...Agilent Technologies Agilent 1260 Infinity Quaternary LC VL System Manual and Quick Reference...
Страница 6: ...6 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference Contents...
Страница 36: ...36 Agilent 1260 Infinity Quaternary LC VL Manual and Quick Reference 1 Introduction Detector...
