Abaxis VetScan HM5, Руководство оператора

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4-2 Running a Sample
4.1 Collecting and Handling Samples
Because sample integrity is essential for accurate test results, always follow the guidelines in this sec-
tion for sample handling.

When collecting a sample, select the largest vein possible, and select the appropriate nee-
dle size for the vein.

If blood stops flowing into the syringe before enough sample is acquired, dispose of that
syringe and attempt a new draw with a new needle, new syringe, and new draw site.

Avoid delays of more than 20 seconds between the venipuncture and the sample transfer
to the EDTA tube, and between filling the tube and mixing. Delays can cause platelet
clumping and clot formation.

To achieve proper mixing and prevent clotting, immediately mix samples by inverting
the tubes 10-15 times (25-30 times with very small samples or in low volume micro-
tubes) Inversion speed should be 2-3 inversions per second.

Mix thoroughly by hand immediately before analysis by gently inverting the tube 10-15
times.
CAUTION: Use only tubes containing potassium EDTA (purple/lavender top)
for CBC analysis.
Note: For multiple tube draws, always fill the tubes in this order:
1) blue top
2) red top or tiger top
3) green top
4) lavender/purple top
Note: For best results, fill the tube with blood to at least half its maximum fill
volume (as marked on the tube).
Note: Do not shake samples! Doing so can damage the blood cells, and can
form micro-bubbles that will cause inaccurate results.