10x Genomics Visium CytAssist, Руководство пользователя

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CG000542 | Rev A
User Guide | CytAssist Instrument | System Operation
Link to TOC | 10xgenomics.com
The run-ready screen is the most common state of the instrument. There
are several key functions accessible directly from the home screen.
Main Operation Overview
This section is meant as a general overview of instrument operation and its placement
within the steps of the Visium CytAssist Spatial Gene Expression assay. When performing
the protocol, follow the steps listed in the appropriate assay kit user guide and use this
section only as a reference.
Before proceeding with the appropriate Visium CytAssist Spatial Gene
Expression assay:
Select two tissue slides containing sections of interest, and verify
that the tissue section is located in an area that results in successful
analyte transfer and imaging. Overlay the slides on the diagrams in the
Visium CytAssist Quick Reference Cards – for Accessory Kit Instruction
(CG000548), located in the Instrument Accessory Kit.
Refer to the Tips & Best
Practices section for further guidance.
If only one tissue on one slide will be loaded, use a blank slide for the
second position on the Tissue Slide Stage.
Follow the protocol in the appropriate assay kit user guide for preliminary
steps and preparation prior to using the Visium CytAssist instrument.
a.
Gather two tissue slides, the appropriate Visium CytAssist Spatial
Gene Expression slide and reagents as listed in the appropriate assay
kit user guide.
Step 4:
Main Operation
Clock
Set up during first
time use
Access through
menu option >
System/Date & Time
Status Icons
Displays connected
devices & network
status
Menu Options
Press icon to
navigate to that
menu option
Run Start Button
Click to begin