1
Zeiss LSM800-6 vertical-2 + Airyscan confocal microscope
2019-03-15
I.
System description:
Inverted (vertical) confocal microscope with Airyscan module
Motorized stage for multiple position imaging
Objectives:
Objective Plan-Apochromat 10x / NA 0.45 Air (WD=2.1mm)
Objective Plan-Apochromat 20x / NA 0.8 Air
Objective W-Plan-Apochromat 40x / NA 1.0 water dipping DIC VIS-IR
Objective C-Apochromat 63x / NA 1.2 W Korr
Laser lines: 405, 488, 561, 640 nm
Detectors:
2 PMT detectors with free choice of the spectral range. (PMT 1: tunable short pass and various
bandpass filters in front of the PMT, PMT 2: tunable long pass and various bandpass filters in
front of detector)
1 GaASP PMT for the Airyscan detector that can function as a normal detector in confocal mode
(detection range adjustable by a tunable short pass and a tunable long pass filter, no bandpass
filters)
An ESID detector for transmitted light.
Simultaneous detection of only two dyes possible, more than two have to be acquired sequentially.
II.
Starting up:
1.
Switch on the multiple socket (1). Provides power to microscope controllers
2.
Switch on the system(2) and Components (3)
3.
Switch on the computer (4)
4.
Login: BIF-User (Useme!11)
5.
Open
ZEN Blue
Software and press “Start System” (“Image Processing” is for offline analysis of
your images)
6.
If Pop-up appears about stage calibration, press “calibrate now”
